Location: Cereal Crops ResearchTitle: Development and Validation of Semi-Thermal Asymmetric Reverse PCR Markers for Ug99-effective Stem Rust Resistance Genes in Wheat
|ZHANG, JINGLUN - North Dakota State University|
|LONG, YUMING - North Dakota State University|
|GILL, BALJEET - North Dakota State University|
|SHARMA, JYOTI - North Dakota State University|
|ZHANG, QIJUN - North Dakota State University|
|CAI, XIWEN - North Dakota State University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/11/2020
Publication Date: 1/11/2020
Citation: Zhang, J., Long, Y., Gill, B.K., Sharma, J.S., Zhang, Q., Klindworth, D.L., Cai, X., Friesen, T.L., Faris, J.D., Xu, S.S. 2020. Development and Validation of Semi-Thermal Asymmetric Reverse PCR Markers for Ug99-effective Stem Rust Resistance Genes in Wheat [abstract]. Plant and Animal Genome Conference XXVII. PO0941.
Interpretive Summary: .
Technical Abstract: The Ug99 race group of the stem rust pathogen (Puccinia graminis f. sp. tritici) is currently considered a serious threat to global wheat production. Identification and deployment of Ug99-effective stem rust resistance (Sr) genes have become major areas of focus in many breeding programs around the world. So far, over 20 Ug99-effective genes have been identified in wheat and its relatives. We recently developed a robust and user-friendly PCR-based method named semi-thermal asymmetric reverse PCR (STARP) for genotyping individual SNPs and indels and initiated a project to develop STARP markers for Ug99-effective genes with high breeding value. We previously developed STARP markers for Sr13, Sr47, Sr8155B1, and two potentially novel genes from an emmer wheat accession. Here, we report the development and validation of diagnostic STARP markers for three Sr13 alleles, Sr39, Sr43, and a new Sr gene recently introgressed from Aegilops markgrafii. Among these new markers, two (Xrwgsnp38 and Xrwgsnp39) were developed based on the functional SNPs of the cloned Sr13 haplotypes R1 (Sr13a), R2 (Sr13b), and R3 (Sr13c). Xrwgsnp38 differentiates between R1/R3 (Sr13a/Sr13c) and R2 (Sr13b), and Xrwgsnp39 differentiates between R1 (Sr13a) and R3 (Sr13c). The two markers (Xrwgsnp38 and Xrwgsnp39), along with the previously developed Sr13 locus-specific STARP marker Xrwgsnp37, have been used extensively for detection of Sr13 alleles in durum and related tetraploid wheat germplasm. The markers for Sr39, Sr43, and the new Sr gene from Ae. markgrafii have been used in marker-assisted deployment of these genes into adapted durum and/or bread wheat germplasm.