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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #370048

Research Project: Integration of Traditional Methods and Novel Molecular Strategies for Improving Disease Resistance and Input-use Efficiency in Peanut

Location: National Peanut Research Laboratory

Title: Identification and Association of Differentially Expressed Disease Resistance (R) gene Candidates Involved in Leaf Spot Resistance in Peanut (Arachis hypogaea L.)

item Dang, Phat
item Lamb, Marshall
item CHEN, CHARLES - Auburn University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/30/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary: Abstract Only.

Technical Abstract: Early (ELS) and late leaf spot (LLS) are fungal diseases that cause severe defoliation and can significantly limit peanut production in the United States and around the world. Breeding for high resistance in peanut has been challenging due to strong genotype by environment interaction. These complex traits are controlled by many major and minor quantitative trait loci (QTLs) involving many genes. Furthermore, chromosome locations of the QTLs varied depending on populations or methods utilized for analysis. The goals of this research were to 1) identify candidate genes for leaf spot resistance, and 2) to associate gene-expression to resistance. Candidate genes identified included TMV resistance protein N-like, PTI1-like tyrosine-protein kinase, pto-interacting protein, cysteine-rich receptor-like protein kinase, and phytosulfokine receptor-like. From qPCR analysis utilizing leaf tissues from leaf spot susceptible and tolerant genotypes in field evaluations, several R genes on chromosomes 5 and 8 were differentially regulated and strongly associated with leaf spot resistance. Candidate R genes located on other chromosomes will be evaluated as well. Gene-expression levels and patterns will be associated with peanut genotypes with leaf spot resistance. This research will facilitate the development of peanut varieties with high leaf spot resistance.