Location: Plant Science ResearchTitle: Transformation of long-lived albino epipremnum aureum ‘golden pothos’ and restoring chloroplast development
|HUNG, CHIU-YUEH - North Carolina Central University|
|ZHANG, JIANHUI - North Carolina Central University|
|BHATTACHARYA, CHAYANIKA - North Carolina Central University|
|LI, HUA - North Carolina Central University|
|KITTUR, FAROOQAHMED - North Carolina Central University|
|OLDHAM, CARLA - North Carolina Central University|
|WEI, XIANGYING - Minjiang University|
|CHEN, JIANJUN - University Of Florida|
|XIE, JIAHUA - North Carolina Central University|
Submitted to: Frontiers in Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/19/2021
Publication Date: 5/12/2021
Citation: Hung, C., Zhang, J., Bhattacharya, C., Li, H., Kittur, F., Oldham, C., Wei, X., Burkey, K.O., Chen, J., Xie, J. 2021. Transformation of long-lived albino epipremnum aureum ‘golden pothos’ and restoring chloroplast development. Frontiers in Plant Science. 12:647507. https://doi.org/10.3389/fpls.2021.647507.
Interpretive Summary: Photosynthesis in higher plants occurs in special organelles called chloroplasts present in cells of leaves and other green tissues. Formation of chloroplasts requires the expression of genes from both the cell nucleus and the organelle itself. Studies of this complex process are greatly limited by available experimental systems. In this study, a tissue culture approach was used to generate albino plants that can be induced to form functional chloroplasts. This system will allow for testing the role of individual genes in chloroplast development and help lay the groundwork for future research on manipulating chloroplasts to facilitate the manufacture of biopharmaceuticals.
Technical Abstract: We created ‘long-lived’ albino plants from Epipremnum aureum by tissue culture and established an efficient transformation system. We also demonstrated that their defective color and dysfunctional chloroplast development could be restored by overexpressing Arabidopsis CHL27, a homolog of EaZIP whose impaired expression is responsible for albino phenotype. These ‘long-lived’ albino plants retain the capacity to develop normal chloroplasts, and thus could be utilized for chloroplast biogenesis and associated biological studies.