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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #369814

Research Project: Management of Priority Legume, Oilseed, Vegetable, Forage Grass, Sugar, Ornamental, and Medicinal Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: Pea seed protein concentration: GWAS approach utilizing Pea Core Plus Collection

item Coyne, Clarice - Clare
item MUGABE, DEUS - Washington State University
item ZHENG, PING - Washington State University
item SMITCHGER, JAMIN - Washington State University
item MA, YU - Washington State University
item MAIN, DORRIE - Washington State University
item McGee, Rebecca

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/30/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Pea (Pisum sativum L.) is a very good source of protein, vitamin K, manganese, dietary fiber, vitamin B1, copper, vitamin C, phosphorus and folate and a good source of vitamin B6, niacin, vitamin B2, molybdenum, zinc, magnesium, iron, potassium and choline. In a growing market segment, producers are paid a premium based on seed protein concentration. Current cultivars average 22% protein over five years in regional trials. The genetic potential for pea seed protein concentration is high, ranging from 31% to 34.4% of seed weight are reported. We assembled a 482 pea accession collection consisting of yellow (cotyledon) pea lines: cultivars, high protein USDA accessions, and USDA pea core accession for a genome wide association study (GWAS) to determine SNPs associated with positive alleles controlling high protein. Genotyping was conducted using genotyping-by-sequencing (GBS) for SNP discovery. A second field study was used to determine the genotype by environment (G x E) interaction on seed protein concentrations of 28 entries current yellow pea cultivars and advanced breeding lines of yellow pea. Results of the SNP discovery and the first year GWAS (1 location, RCBD) and G x E (3 locations, RCBD) field studies will be reported.