|DAI, Z - South China Agricultural University|
|WU, F - South China Agricultural University|
|ZHENG, Z - South China Agricultural University|
|Yokomi, Raymond - Ray|
|KUMAGAI, L - California Department Of Food And Agriculture|
|CAI, W - Animal And Plant Health Inspection Service (APHIS)|
|RASCOE, J - Animal And Plant Health Inspection Service (APHIS)|
|DENG, Z - South China Agricultural University|
Submitted to: International Research Conference on Huanglongbing
Publication Type: Abstract Only
Publication Acceptance Date: 2/8/2019
Publication Date: 3/10/2019
Citation: Dai, Z., Wu, F., Zheng, Z., Yokomi, R.K., Kumagai, L., Cai, W., Rascoe, J., Polek, M., Deng, Z. 2019. “Candidatus Liberibacter asiaticus” strains from multiple locations in southern California are different. International Research Conference on Huanglongbing, March 10-15, 2019, Riverside, California.
Technical Abstract: “Candidatus Liberibacter asiaticus” (CLas) was first found in Florida in 2005 and is now endemic there. In California, CLas was first detected in Hacienda Heights in Los Angeles County in 2012 and has now been detected in multiple urban locations in southern California. Knowledge of CLas strain diversity in California is important for HLB management. In this study, genomic diversity among ten CLas strains from six California locations were analyzed using next generation sequencing (Illumina MiSeq and HiSeq) approach. Draft genome sequences of CLas strains were assembled. Sequences of 16S rRNA gene and nrdB confirmed CLas identity. Prophages were detected in all CLas strains. The California strains formed four prophage typing groups (PTGs): PTG1 with Type 1 prophage only (strains from Anaheim, San Gabriel, and Riverside); PTG2 with Type 2 prophage only (strains from Hacienda Heights); PTG1-3 with both Type 1 and 3 prophages (a strain from Cerritos); and PTG1-2 with both Type 1 and Type 2 prophages (a strain from La Habra). Analyses of terL sequence showed that all California CLas strains were more related to strains in Asia, rather than those from Florida. Miniature inverted-repeat transposable elements (MITEs) were found in all CLas strains. Altogether, the NGS approach in this study revealed diversity of CLas strains in California which indicated independent introductions at multiple times.