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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #368893

Research Project: Intestinal Microbial Ecology and Metagenomic Strategies to Reduce Antibiotic Resistance and Foodborne Pathogens

Location: Food Safety and Enteric Pathogens Research

Title: Successful development of methodology for detection of hapten-specific contact hypersensitivity (CHS) memory in swine

Author
item PUTZ, ELLIS - Iowa State University
item PUTZ, AUSTIN - Iowa State University
item BOETTCHER, ADELINE - Iowa State University
item CHARLEY, SARA - Iowa State University
item SAUER, MARY - Iowa State University
item Palmer, Mitchell
item PHILLIPS, RACHEL - Iowa State University
item HOSTETTER, JESSE - Iowa State University
item Loving, Crystal
item CUNNICK, JOAN - Iowa State University
item TUGGLE, CHRISTOPHER - Iowa State University

Submitted to: PLoS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/24/2019
Publication Date: 10/9/2019
Citation: Putz, E.J., Putz, A.M., Boettcher, A., Charley, S., Sauer, M., Palmer, M.V., Phillips, R., Hostetter, J.M., Loving, C.L., Cunnick, J.E., Tuggle, C.K. 2019. Successful development of methodology for detection of hapten-specific contact hypersensitivity (CHS) memory in swine. PLoS One. 14(10):e0223483. https://doi.org/10.1371/journal.pone.0223483.
DOI: https://doi.org/10.1371/journal.pone.0223483

Interpretive Summary: Pigs are often used as an animal model for human research due to the similarites between human and pig organs and function. Contact hypersensitivity (CHS) to different compounds occurs in humans and animals, and while rodent models can be informative, non-rodent models of CHS are needed to better evaluate mechanism and interventsion. There are two main categories of the immune system, referred to as innate and adaptive. Innate immunity are methods of defense conserved across vetebrates, invertebrates and even plants. CHS responses include activation of innate immunity, and are typically responses against chemical compounts. A CHS model was developed and tested in pigs, and results indicate that both innate and adaptive components of the immunce system play a role in the second exposure response. Data provide a useful protocol for futher testing of mechanism and potential intervention strategies in a pig model.

Technical Abstract: Hapten contact hypersensitivity (CHS) elicits a well-documented inflammation response that can be used to illustrate training of immune cells through hapten-specific CHS memory. The education of hapten-specific memory T cells has been established, recent research in mice has expanded the “adaptive” characteristic of a memory response from solely a function of the adaptive immune system, to innate cells as well. To test whether similar responses are seen in a non-rodent model, we used hapten-specific CHS to measure the ear inflammation response of outbred pigs to dinitrofluorobenzene (DNFB), oxazolone (OXA), or vehicle controls. We adapted mouse innate memory literature protocols to the domestic pig model. Animals were challenged up to 32 days post initial sensitization exposure to the hapten, and specific ear swelling responses to this challenge were significant for 7, 21, and 32 days post-sensitization. We established hapten-specific CHS memory exists in a non-rodent model. We also developed a successful protocol for demonstrating these CHS responses in a porcine system.