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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #368461

Research Project: Biological, Genetic and Genomic Based Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Expression of Ribosome-inactivating Protein Is Enhanced in Watermelon with Resistance to Papaya Ringspot Virus

Author
item Chanda, Bidisha
item WU, SHAN - Boyce Thompson Institute
item FEI, ZHANGJUN - Boyce Thompson Institute
item Ling, Kai-Shu
item Levi, Amnon

Submitted to: Plants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/13/2021
Publication Date: 1/20/2021
Citation: Bidisha Chanda, Shan Wu, Zhangjun Fei, Kai-Shu Ling & Amnon Levi. 2022. Elevated expression of ribosome-inactivating protein (RIP) genes in potyvirus-resistant watermelon in response to viral infection. Canadian Journal of Plant Pathology. Published on Line
DOI: https://doi.org/10.1080/07060661.2021.2021450

Interpretive Summary: Watermelon, originating in Africa, is an important vegetable fruit crop widely grown in the U.S. and around the world. Besides the water content, watermelon is considered to be a rich source of vitamins, antioxidants and amino acids. One of major limiting factors in watermelon crop production is viral diseases. Several aphid-borne potyviruses, including papaya ringspot virus (PRSV) and zucchini yellow mosaic virus (ZYMV), have caused serious economic losses to watermelon production. Previously, we determined that a translation elongation factor (eIF4E) is responsible in conferring watermelon with resistance to ZYMV. Other studies have shown that ribosome-inactivating proteins (RIPs) have anti-microbial, antiviral and insecticidal properties. Watermelon and other cucurbits have been shown to contain a high level of RIPs. In the present study, we were interested in evaluating RIP expression in association with PRSV infection on watermelon. Interestingly, a higher level of RIP expression was associated with PRSV resistant watermelon plants. This finding may offer us an additional bio-marker in identifying watermelon with broad spectrum disease resistance.

Technical Abstract: Ribosome-inactivating proteins (RIPs) are widely known for their antiviral activity. We report RIPs from watermelon Citrullus lanatus subsp. vulgaris cv. Charleston Gray to be associated with resistance to Papaya ringspot virus watermelon strain (PRSV-W). PRSV-W, a potyvirus, causes infection to all types of cucurbits resulting in severe economic losses, similar to that of a related strain on papaya (PRSV-P) that has devastated the papaya industry worldwide. In our previous studies, we determined that a single nucleotide polymorphism in the watermelon eIF4E is likely responsible for recessive resistance in watermelon against a potyvirus, zucchini yellow mosaic virus (ZYMV). In the present study, using watermelon cultivars with distinct phenotype of resistance and susceptibility to PRSV-W infection and comparative measurement of expression of RIPs in watermelon throughout a disease progression process, we identified an enhanced expression of RIPs in PRSV-resistant watermelon upon PRSV-W infection.