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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #368457

Research Project: Combining Phenotype, Genetics, and Genomics to Develop Common Beans with Resistance to Highly Variable Pathogens

Location: Soybean Genomics & Improvement Laboratory

Title: Mapping and marker development for Ur-5 rust resistance locus

item Hurtado-Gonzales, Oscar
item VALENTINI, GISELI - Universidade Estadual De Maringá
item Song, Qijian
item Pastor Corrales, Marcial - Talo

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Trade Journal
Publication Acceptance Date: 2/25/2019
Publication Date: 4/30/2019
Citation: Hurtado-Gonzales, O.P., Valentini, G., Song, Q., Pastor Corrales, M.A. 2019. Mapping and marker development for Ur-5 rust resistance locus. Bean Improvement Cooperative Annual Report. 62:25-26.

Interpretive Summary: Breeding for disease resistance is the most effective strategy for managing the bean rust disease, a major problem in Central American and African countries. USDA-ARS scientists in Beltsville, MD, are working to identify rust resistance genes using DNA-based marker technology that accurately identifies a gene. These markers will allow the rapid breeding of the resistance genes into elite bean clutivars grown by farmers. It is expected that the marker technology and newly bred beans will help increase bean yield and/or reduce costs associated with the control of the rust disease of common bean.

Technical Abstract: Several molecular markers have been developed over the past 25 years tagging the Ur-3, Ur-4, Ur-5, Ur-6, Ur-7, Ur-11, Ur-13, and Ur-14 rust resistance genes. The BIC website includes a comprehensive list of rust resistance DNA markers together with the primer sequences and PCR conditions. The accuracy of some of these markers is limited due to the recombination between the markers and the resistance loci. Thus, there is a need to develop more accurate and tightly linked markers that can be used in a high-throughput approach avoiding the gel-based labor intensive methods. The Mesoamerican Ur-5 rust resistance locus provides resistance to more than 60 out of 88 races of Uromyces appendiculatus, maintained at ARS-Beltsville. Pyramiding the Ur-5 locus with other rust resistance loci can be difficult due to epistatic interactions. Currently, the most reliable method of detection for the presence of Ur-5 locus is using multiple races of the rust pathogen. The objective of this study was to develop and validate an Ur-5 SNP-based marker that can reliable be used in breeding programs to develop common bean cultivars with Ur-5.