|Hinton, Jr, Arthur|
|Cox, Nelson - Nac|
|LEVICAN, ARTURO - Departamento De Ingeniería Hidráulica Y Ambiental, Pontificia Universidad Cato´lica De Chile|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/3/2019
Publication Date: 9/8/2019
Citation: Hinton Jr, A., Cox Jr, N.A., Levican, A. 2019. Formulation of a Selective Medium for Aerobic Recovery of Campylobacter. Meeting Abstract. CHRO 2019 Book of Abstracts.
Interpretive Summary: none
Technical Abstract: Problem Statement: A selective medium for isolating Campylobacter from mixed bacteria cultures under aerobic incubation would simplify procedures for recovering this pathogen from environmental samples. Approach: A non-selective basal medium was autoclaved, cooled, and supplemented with sterile sodium bicarbonate. The selective medium was prepared by adding the Bolton antibiotic mixture to the basal medium. Basal and selective media were transferred to 25 ml culture flasks. Mixed cultures were prepared by adding either Campylobacter fetus, Campylobacter coli, Campylobacter jejuni, or Campylobacter lari to bacterial suspensions containing Enterococcus faecalis, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa Salmonella Kentucky, and Staphylococcus aureus. Separate culture flasks containing the basal medium or the selective medium were inoculated with the mixed cultures, then incubated aerobically at 37C for 48 h. After incubation, isolates were enumerated on the appropriate selective, differential bacterial agar medium. Results: Results indicated that all isolates were recovered from basal medium after aerobic incubation and that significantly (p < 0.05) fewer Campylobacter spp. were recovered than all other isolates, except S. aureus, in the mixed culture. However, after incubation in media supplemented with the Bolton antibiotic mixture, significantly more of each Campylobacter isolate was recovered than the other bacterial isolates. Furthermore, there was no significant growth of the other bacteria in the mixed culture, and no E. coli, Salmonella Kentucky, or S. aureus were recovered in most experiments. Conclusions: Supplementing the basal medium with the Bolton mixture produces a selective medium that can be incubated aerobically to isolate Campylobacter from bacterial cultures.