|Cox, Nelson - Nac|
|SHARIAT, NIKKI - Gettysburg College|
|Cook, Kimberly - Kim|
|Meinersmann, Richard - Rick|
Submitted to: International Association for Food Protection Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/27/2019
Publication Date: 7/21/2019
Citation: Berrang, M.E., Cox Jr, N.A., Shariat, N., Cook, K.L., Frye, J.G., Meinersmann, R.J. 2019. Detection of multiple serotypes of Salmonella on pre-Chill chicken carcasses by whole carcass rinse or whole carcass incubation [abstract]. International Association for Food Protection Proceedings. 98(1):63.
Interpretive Summary: Interpretive summary not required for a meeting abstract
Technical Abstract: Chicken carcasses and meat products may be contaminated with Salmonella and have been implicated in disease outbreaks. Generally, only one or two Salmonella colonies are characterized from any one positive carcass; it is not clear how many serotypes of Salmonella may be present. The objective of this study was to compare the number of Salmonella serotypes detected from pre-chill chicken carcasses sampled by standard carcass rinse to those sampled by whole carcass incubation. Eight broiler carcasses, four from each of two flocks, were collected pre-chill at a commercial slaughter plant. Each carcass was subjected to a whole carcass rinse in 400mL buffered peptone water (BPW), an aliquot was removed for incubation and the carcass was incubated in the remaining fluid. Plating was conducted on BG-sulfa, XLT4 and Hectoen Enteric agars before and after BPW pre-enrichment and after selective enrichment in RV, TT or RV to TT broths. Up to three typical Salmonella colonies were picked from each plate from each broth (potential 45 picks per sample) and characterized by serogrouping and Smart PCR serotyping. Serotypes detected from 268 total picks included: Kentucky, Typhimurium, Schwarzengrund, Enteritidis and Hadar. On average, significantly more (P<0.05) serotypes were detected by whole carcass enrichment than from rinse of the same carcass (3 vs 1.9 respectively). Schwarzengrund was the only serotype detected significantly more often from whole carcass enrichment than from rinse samples (7 vs 2 respectively). Sampling chicken by analysis of a carcass rinse aliquot may limit the diversity of serotypes detected. Tightly bound salmonellae may be more likely detected by incubation of the whole carcass.