Location: Characterization and Interventions for Foodborne Pathogens
Title: Inkjet printed electrochemical aptasensor for detection of Hg2+ in organic solventsAuthor
DIAZ-AMAYA, SUSANA - Purdue University | |
LIN, LI KAI - Purdue University | |
DININO, RENEE - Purdue University | |
OSTOS, CARLOS - Purdue University | |
STANCIU, LIA - Purdue University |
Submitted to: Electrochemical Acta
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 5/15/2019 Publication Date: 5/22/2019 Citation: Diaz-Amaya, S., Lin, L., Dinino, R.E., Ostos, C., Stanciu, L.A. 2019. Inkjet printed electrochemical aptasensor for detection of Hg2+ in organic solvents. Electrochemical Acta. 316:33-42. https://doi.org/10.1016/j.electacta.2019.05.079. DOI: https://doi.org/10.1016/j.electacta.2019.05.079 Interpretive Summary: The increasing incidence of infectious outbreaks from contaminated food and water supply continues imposing a global burden for food safety, creating a market demand for on-site, disposable, easy-to-use, and cost-efficient devices. This study demonstrates a cost-efficient, reliable, and repeatable color-changing sensor for the detection of pathogenic E. coli O157:H7 from ground beef. The sensors were generated by printing bioactive inks containing DNA molecules (aptamers) that specifically bind the pathogenic bacteria. Binding of the pathogenic E. coli to the printed sensor causes a color change the intensity of which is corelated to the amount of pathogen present. The repeatability of the test was demonstrated by the ability to control the variation within one standard deviation for the majority of the sensors and even at low concentrations. This study demonstrates a rapid and reliable method to identify low concentration of pathogens in food and could be used to improve public health by preventing outbreaks of foodborne illness. Technical Abstract: The increasing incidence of infectious outbreaks from contaminated food and water supply continues imposing a global burden for food safety, creating a market demand for on-site, disposable, easy-to-use, and cost-efficient devices. Despite of the rapid growth of biosensors field and the generation of breakthrough technologies, more than 80% of the platforms developed at lab-scale never will get to meet the market. This work aims to provide a cost-efficient, reliable, and repeatable approach for the detection of foodborne pathogens in real samples. For the first time an optimized inkjet printing platform is proposed taking advantage of a carefully controlled nanopatterning of novel carboxyl-functionalized aptameric ink on a nitrocellulose substrate for the highly efficient detection of E. coli O157:H7 (25 colony forming units (CFU) mL-1 in pure culture and 233 CFU mL-1 in ground beef ) demonstrating the ability to control the variation within +/- 1 SD for at least 75% of the data collected even at very low concentrations. From the best of the knowledge this work reports the lowest limit of detection of the state of the art for paper-based optical detection of E. coli O157:H7, with enough evidence (p > 0.05) to prove its high specificity at genus, species, strain, and serotype level. |