Location: Corn, Soybean and Wheat Quality ResearchTitle: Differential distributions of trafficking and signaling proteins of the maize ER-Golgi apparatus
|OKEKEOGBU, IKENNA - Purdue University|
|ARYAL, UMA - Purdue University|
|GONZALEZFERNANDEZ, SUSANA - Lawrence Berkeley National Laboratory|
|HEAZLEWOOD, JOSHUA - Lawrence Berkeley National Laboratory|
|MCCANN, MAUREEN - Purdue University|
|CARPITA, NICHOLAS - Purdue University|
Submitted to: Plant Signaling and Behavior
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/20/2019
Publication Date: 9/28/2019
Citation: Okekeogbu, I.O., Aryal, U.K., Gonzalez Fernandez-Nino, S.M., Penning, B., Heazlewood, J.L., McCann, M.C., Carpita, N.C. 2019. Differential distributions of trafficking and signaling proteins of the maize ER-Golgi apparatus. Plant Signaling and Behavior. 14(12):1672513. https://doi.org/10.1080/15592324.2019.1672513.
Interpretive Summary: In plants, the Golgi apparatus transports proteins to the cell wall for different functions. The function of those proteins are not always known. Some of those proteins act in cell to cell signaling or as targets to move vesicles containing other materials to the cell wall or the plasma membrane. We identified many proteins transported to the cell wall involved in cell to cell signaling or acting as potential targets for the moving of other material to the cell wall or plasma membrane in vesicles. Flotation centrifugation followed by free-flow electrophoresis was used to separate out Golgi into different groups that were responsible for moving different proteins for different jobs. Using this system, we split the Golgi in four different groups finding we could better associate identified proteins with specific classifications within each of these four groups. This system allows for multiple fractions of Golgi to be separated by function based on known proteins and will aid scientists in defining functions of unknown proteins found in the same Golgi fractions. Knowledge of what and when things are transported to the cell wall from Golgi will aid in any trait or industry which involves plant cell walls such as: bioenergy, bioproducts, physical pathogen resistance, or quality of baked goods from whole wheat.
Technical Abstract: The ER-Golgi apparatus of plants is the site of synthesis of non-cellulosic polysaccharides that then traffic to the cell wall. A two-step protocol of flotation centrifugation followed by free-flow electrophoresis (FFE) resolved ER and Golgi proteins into three profiles: an ER-rich fraction, bulk Golgi-rich fraction, and intermediate fraction enriched in cellulose synthases. Nearly three dozen Rab-like proteins of eight different subgroups were distributed differentially in ER- vs. Golgi-rich fractions, whereas seven 14-3-3 proteins co-fractionated with cellulose synthases in the intermediate fraction. FFE offers a powerful means to classify resident and transient proteins in cell-free assays of cellular location.