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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #366725

Research Project: Genetics, Epigenetics, Genomics, and Biotechnology for Fruit and Vegetable Quality

Location: Plant, Soil and Nutrition Research

Title: The Ptr1 locus of Solanum lycopersicoides confers resistance to race 1 strains of Pseudomonas syringae pv. tomato and to Ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN

Author
item MAZO-MOLINA, CAROLINA - Boyce Thompson Institute
item MAINIERO, SAMANTHA - Boyce Thompson Institute
item HIND, SARA - Boyce Thompson Institute
item KRAUS, CHRISTINE - Boyce Thompson Institute
item VACHEV, MISHI - Boyce Thompson Institute
item MAVIANE-MACIA, FELICIA - University Of Toulouse
item LINDEBERG, M. - Cornell University - New York
item SAHA, SURYA - Boyce Thompson Institute
item STRICKLER, SUSAN - Boyce Thompson Institute
item FEDER, ARI - Boyce Thompson Institute
item Giovannoni, James
item SMART, CRISTINE - Cornell University - New York
item PEETERS, NEMO - University Of Toulouse
item MARTIN, GREG - Cornell University - New York

Submitted to: Molecular Plant-Microbe Interactions
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/17/2019
Publication Date: 6/15/2019
Citation: Mazo-Molina, C., Mainiero, S., Hind, S., Kraus, C., Vachev, M., Maviane-Macia, F., Lindeberg, M., Saha, S., Strickler, S., Feder, A., Giovannoni, J.J., Smart, C., Peeters, N., Martin, G. 2019. The Ptr1 locus of Solanum lycopersicoides confers resistance to race 1 strains of Pseudomonas syringae pv. tomato and to Ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN. Molecular Plant-Microbe Interactions. 32(8):040-960. https://doi.org/10.1094/MPMI-01-19-0018-R.
DOI: https://doi.org/10.1094/MPMI-01-19-0018-R

Interpretive Summary: Bacterial speck disease of tomato occurs in cool, wet environments that favor bacterial spread. Two races of bacterial speck, 0 and 1, are currently defined which differ in their ability to cause disease on tomato varieties carrying the resistance gene Pto. Race 1 strains which causes bacterial speck disease of tomato even in the presence of this resistance gene, are becoming increasingly common and no simply-inherited genetic resistance to such strains is known. Here we identified and characterized race 1 resistance in a wild tomato relative. This natural genetic resistance in a wild tomato presents and opportunity to breed for resistance to this increasingly prevalent tomato disease. This should result in both improved tomato yield and reduced pesticide application.

Technical Abstract: We discovered that a S. lycopersicoides locus, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 Pst strains by recognizing the type III effector AvrRpt2. In Arabidopsis, AvrRpt2 degrades the RIN4 protein thereby activating RPS2-mediated immunity. Ptr1 also recognized homologs of AvrRpt2 from diverse bacteria including one in Ralstonia pseudosolanacearum and this correlated with AvrRpt2 ability to degrade RIN4. Using site-directed mutagenesis of AvrRpt2 we found that Ptr1 and RPS2 recognize identical features of AvrRpt2. However, the S. lycopersicoides genome sequence revealed no RPS2 homolog in the Ptr1 region. Ptr1 could play an important role in controlling bacterial speck disease and its future cloning may shed light on an example of convergent evolution for recognition of a widespread type III effector.