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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Cotton Structure and Quality Research » Research » Publications at this Location » Publication #366720
Research Project: Improved Quality Assessments of Cotton from Fiber to Final Products

Location: Cotton Structure and Quality Research

Title: Use of ATR FT-IR spectroscopy in identification of immature fiber (im) mutant and Texas Marker-1 (TM-1) cotton fibers

Author
item Liu, Yongliang
item Kim, Hee

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/20/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The immature fiber (im) mutant is one type of cotton fiber mutant with unique characteristics of non-fluffy cotton bolls. Compared to its near-isogenic line (NIL) wild type Texas Marker-1 (TM-1), im fiber has a thin secondary cell wall and is less mature. In this study, we applied the previously proposed simple algorithms (i.e., infrared maturity and crystallinity index) to analyze the attenuated total reflection Fourier transform infrared (ATR FT-IR) spectra for potential identification of im fibers from TM-1 fibers grown in the field over four years (2007, 2011, 2014, and 2015). Result of plotting infrared maturity values versus infrared crystallinity values showed a clear pattern of separation between im fibers and TM-1 fibers. Because ATR FT-IR method enables the measurement of fiber maturity and crystallinity properties on small amount of breeder’s fiber samples in a rapid and non-destructive way, it offers an alternative way of phenotyping cotton fibers and can help geneticists and breeders interpret their genetic and genomic data.