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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Endemic Poultry Viral Diseases Research » Research » Publications at this Location » Publication #366223

Research Project: Enhancing Genetic Resistance to Marek’s Disease in Poultry

Location: Endemic Poultry Viral Diseases Research

Title: Genome-wide characterization of copy number variations in the host genome in genetic resistance to Marek’s disease using next generation sequencing

Author
item BAI, HAO - Yangzhou University
item HE, YANGHUA - University Of Maryland
item DING, YI - University Of Maryland
item CHU, QIN - University Of Maryland
item LIAN, LING - China Agriculture University
item HEIFETZ, ELIYAHU - Jerusalem College Of Technology
item YANG, NING - China Agriculture University
item Cheng, Hans
item Zhang, Huanmin
item CHEN, JILAN - Chinese Academy Of Agricultural Sciences
item SONG, JIUZHOU - University Of Maryland

Submitted to: BMC Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/5/2020
Publication Date: 7/16/2020
Citation: Bai, H., He, Y., Ding, Y., Chu, Q., Lian, L., Heifetz, E.M., Yang, N., Cheng, H.H., Zhang, H., Chen, J., Song, J. 2020. Genome-wide characterization of copy number variations in the host genome in genetic resistance to Marek’s disease using next generation sequencing. BMC Genetics. 21:77. https://doi.org/10.1186/s12863-020-00884-w.
DOI: https://doi.org/10.1186/s12863-020-00884-w

Interpretive Summary: Marek’s disease (MD) is an infectious disease of chickens and remains as a threat to the poultry industry. MD has been primarily controlled by vaccination, which, in combination with required managemental measures and production losses at slaughter, incurs a sizable annual expense to the industry. Therefore, understanding why some chickens are resistance to MD is of great importance in control of the disease. Copy number variation (CNV) is a type of DNA variation detectable in both human and animal genomes. This study has identified significant differences in CNV between a genetic line of chickens that is known resistant to MD and another line known with high susceptibility. Further analyses also showed that some of the CNVs are involved in important biological functions. These findings provided valuable insights for advancing the basic understanding on potential genetic mechanism that control genetic resistance to MD, which would eventually benefit the poultry industry in control of this infectious disease in chicken.

Technical Abstract: Marek’s disease (MD) is a neoplastic disease of chickens and remains as a threat to the poultry industry. MD has been primarily controlled by vaccination, which, in combination with required control measures and production losses at slaughter, incurs a sizable annual expense to the industry. Therefore, genetic resistance to MD offers an attractive augment in control of the disease. Copy number variation (CNV), a phenomenon in which sections of the genome are repeated and the number of repeats in the genome varies between individuals in the human population as well as in animals, were examined in highly inbred lines and hybrid groups of chickens, which vary in genetic resistance to MD. This genome-wide CNV detection resulted a total of 1,649 CNV regions (CNVRs). Ninety of the CNVRs were in common across all the chicken lines/strains/groups. Fifty-five and forty-four CNVRs were specifically identified in two highly inbred lines of chickens, respectively. One of the inbred lines is known highly resistance to MD and the other, highly susceptible. Further analyses also shown some of the CNVRs are involved in key biological processing functions. These findings provided valuable insights for advancing the basic understanding on potential genetic mechanism conferring resistance to MD, which would eventually benefit the poultry industry in control of this infectious disease in chicken.