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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Publications at this Location » Publication #365557

Research Project: Mitigating High Consequence Domestic, Exotic, and Emerging Diseases of Fruits, Vegetables, and Ornamentals

Location: Subtropical Plant Pathology Research

Title: Novel insight into the distribution and dissemination of CandidatusLiberibacter asiaticus, the causal agent ofcitrus Huanglongbing

Author
item WANG, QINGLIAN - Huazhong Agricultural University
item XU, YUNLONG - Huazhong Agricultural University
item YANG, XIAOFEN - Huazhong Agricultural University
item JIA, JIN - Huazhong Agricultural University
item ZHOU, JIALE - Huazhong Agricultural University
item Duan, Ping
item Hartung, John
item DING, FANG - Huazhong Agricultural University

Submitted to: Plant Biotechnology Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/2/2021
Publication Date: 11/20/2021
Citation: Wang, Q., Xu, Y., Yang, X., Jia, J., Zhou, J., Duan, Y., Hartung, J.S., Ding, F. 2021. Novel insight into the distribution and dissemination of CandidatusLiberibacter asiaticus, the causal agent ofcitrus Huanglongbing. Plant Biotechnology Journal. 20, pp. 247–249. https://doi.org/10.1111/pbi.13753.
DOI: https://doi.org/10.1111/pbi.13753

Interpretive Summary: Citrus huanglongbing (HLB) is currently the most devastating citrus disease worldwide. Diagnosis of HLB prior to the development of symptoms remains challenging. In this study, we prepared a synthetic peptide, derived from the amino acid sequence of an effector protein of ‘Candidatus Liberibacter asiaticus’ (CLas), and developed a simple and direct tissue blot immuno assay for specific recognition of CLas bacteria in HLB-affected plants. This technique is simple, sensitive and robust. This antibody can be further developed as a test paper to provide practical and rapid high throughput diagnosis of Huanglongbing to meet the current urgent needs of global citrus industry.

Technical Abstract: Citrus huanglongbing (HLB) is currently the most devastating citrus disease and has caused enormous economic losses to many citrus growing regions of the world. Diagnosis of HLB prior to the development of symptoms remains challenging. In this study, we prepared a synthetic peptide, CISRTRIDSSPPPGH, derived from the amino acid sequence of CLIBASIA_05315 (CLas-05315), which is a secreted effector protein of ‘Candidatus Liberibacter asiaticus’ (CLas). We optimized assay conditions to enable specific recognition of CLas bacteria in a simple and direct tissue blot immuno assay (DTBIA). The technique developed in this study is simple, sensitive and robust. This antibody is a promising candidate for the development of a test paper to provide practical and rapid high throughput diagnosis of Huanglongbing to meet the current urgent needs of global citrus industry.