Location: Produce Safety and Microbiology ResearchTitle: New Strategies for the Enumeration of Enteric Pathogens in Water
Submitted to: Environmental Microbiology Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/21/2019
Publication Date: 7/25/2019
Citation: Gorski L, Rivadeneira P, Cooley MB. 2019. New strategies for the enumeration of enteric pathogens in water. Environmental Microbiology. 11(6):765-776. https://doi.org/10.1111/1758-2229.12786.
Interpretive Summary: This chapter explains three methods used to determine serotype in the foodborne pathogen Listeria monocytogenes. The chapter provides detailed, step-by-step materials and methods for the agglutination method, the enzyme linked immunosorbent assay method, and the polymerase chain reaction method.
Technical Abstract: For assessing isolates of Listeria monocytogenes, serotype designation is the first subtyping method used. Traditionally, serotyping has been done with agglutination reactions; however, alternative methods utilizing Enzyme Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) are common. Described here are the three methods, and the advantages and disadvantages of each.