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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #364936

Research Project: Ecology and Detection of Human Pathogens in the Produce Production Continuum

Location: Produce Safety and Microbiology Research

Title: New Strategies for the Enumeration of Enteric Pathogens in Water

item Gorski, Lisa

Submitted to: Environmental Microbiology Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/21/2019
Publication Date: 7/25/2019
Citation: Gorski L, Rivadeneira P, Cooley MB. 2019. New strategies for the enumeration of enteric pathogens in water. Environmental Microbiology. 11(6):765-776.

Interpretive Summary: This chapter explains three methods used to determine serotype in the foodborne pathogen Listeria monocytogenes. The chapter provides detailed, step-by-step materials and methods for the agglutination method, the enzyme linked immunosorbent assay method, and the polymerase chain reaction method.

Technical Abstract: For assessing isolates of Listeria monocytogenes, serotype designation is the first subtyping method used. Traditionally, serotyping has been done with agglutination reactions; however, alternative methods utilizing Enzyme Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) are common. Described here are the three methods, and the advantages and disadvantages of each.