Location: Plant Science ResearchTitle: Quantifying MAMP-induced production of reactive oxygen species in sorghum and maize
|SAMIRA, ROZALYNNE - North Carolina State University|
|ZHANG, XINYE - North Carolina State University|
|KIMBALL, JENNIFER - University Of Missouri|
|CUI, YAYA - University Of Missouri|
|STACEY, GARY - University Of Missouri|
Submitted to: Bio-protocol
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/1/2019
Publication Date: 7/20/2019
Citation: Samira, R., Zhang, X., Kimball, J., Cui, Y., Stacey, G., Balint Kurti, P.J. 2019. Quantifying MAMP-induced production of reactive oxygen species in sorghum and maize. Bio-protocol. https://doi.org/10.21769/BioProtoc.3304.
Interpretive Summary: We describe methods to measure the basal defense response in maize and sorghum.
Technical Abstract: Plants have the ability to recognize microbe associated molecular profiles (MAMPS) and mount a defense response. The level of the MAMP response can vary depending on genetic and environmental factors. The most commonly studied MAMPs are flg22, an epitope from bacterial flagellin, and chitin, a component of the fungal cell wall. Protocols for measuring ROS production elicited by flg22 and chitin in maize and sorghum are described.