|BEECHER, BRIAN - Grain Inspection, Packers & Stockyards Administration (GIPSA, USDA)|
Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/25/2019
Publication Date: 5/22/2020
Citation: Morris, C.F., Kiszonas, A., Beecher, B.S., Peden, G.L. 2020. Registration of six partial waxy-near-isogenic hexaploid wheat genetic stock lines lacking one or two granule bound starch synthase genes. Journal of Plant Registrations. 14:217-220. https://doi.org/10.1002/plr2.20010.
Interpretive Summary: Starch is the leading component of wheat grain and flour. Starch is comprised primarily of amylose and amylopectin and is deposited in the endosperm as semi-crystalline granules with a bimodal size distribution. Amylose is synthesized by Granule Bound Starch Synthase I (GBSS) (E.C. 24.1.21) which occurs in T. aestivum L. as a triplicated homoeologous gene series, Wx-A1, Wx-B1, and Wx-D1, and located on chromosomes 7A, 4A, and 7D, respectively. Naturally occurring mutations in these genes have been described and pyramided to produce full waxy lines. Six GBSS allele near-isogenic line (NIL) hexaploid wheat (T. aestivum L.) genetic stocks were developed at the USDA-ARS Western Wheat Quality Laboratory, Pullman, Washington.
Technical Abstract: Starch is the leading component of wheat (Triticum spp.) grain and flour. Six hexaploid wheat (T. aestivum L.) near-isogenic lines (NILs), WQL19WX7A, WQL19WX4A, WQL19WX7D, WQL19WX7A.4A, WQL19WX7A.7D, WQL19WX4A.7D, were developed to facilitate studies examining the role of starch amylose:amylopectin on wheat processing and end-product quality, and as parents to develop reduced amylose germplasm. Each NIL lacks a functional copy of one or two of the Waxy Granule Bound Starch Synthase I (GBSS) genes, and conversely possesses one or two functional genes. These six genetic stocks were produced in cv. Alpowa soft white spring wheat (PI 566595), and comprise extensions of an on going series of NILs in this wheat cultivar. The NILs are BC7 (Alpowa/Waxy-Pen/7*Alpowa) full sibs, selected among segregating BC7 progeny using allele-specific primers. The GBSS null alleles were derived from the waxy cultivar Waxy-Pen (PI 637779) (Morris and King, 2007). These NILs are complemented by wild-type Alpowa (three functional GBSS genes). These lines were developed at the USDA Agricultural Research Service’s Western Wheat Quality Laboratory, and will be useful to study starch composition and its effects on processing and food product quality, and can serve as parents in developing cultivars with altered starch composition.