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ARS Home » Southeast Area » Miami, Florida » Subtropical Horticulture Research » Research » Publications at this Location » Publication #362685

Research Project: Conservation, Evaluation, and Distribution of Sugarcane, Mango, Avocado and Other Subtropical and Tropical Genetic Resources and Associated Data

Location: Subtropical Horticulture Research

Title: The avocado genome enlightens deep angiosperm phylogeny and reveals pathogen-influenced gene space

Author
item RENDON-ANAYA, MARTHA - National Laboratory Of Genomics And Biodiversity
item IBARRA-LACLETTE, ENRIQUE - National Laboratory Of Genomics And Biodiversity
item MENDEZ-BRAVO, ALFONSO - National Laboratory Of Genomics And Biodiversity
item LAN, TIANYING - University Of Buffalo
item ZHENG, CHUNFANG - University Of Ottawa
item CARRETERO-PAULET, LORENZO - Flanders Institute Of Biotechnology (VIB)
item PEREZ-TORRES, CLAUDIA ANAHI - National Laboratory Of Genomics And Biodiversity
item CHACON-LOPEZ, ALEJANDRA - National Laboratory Of Genomics And Biodiversity
item HERNANDEZ-GUZMAN, GUSTAVO - National Laboratory Of Genomics And Biodiversity
item CHANG, TIEN-HAO - University Of Buffalo
item FARRE, KIMBERLY - University Of Buffalo
item BARBAZUK, W. BRAD - University Of Florida
item CHAMALA, SRIKAR - University Of Florida
item MUTWIL, MAREK - Nanyang Technological University
item SHIVHARE, DEVENDRA - Nanyang Technological University
item MITTER, NEENA - University Of Queensland
item HAYWARD, ALICE - University Of Queensland
item FLETCHER, STEPHEN - University Of Queensland
item ROZAS, JULIO - University Of Barcelona
item SANCHEZ-GRACIA, ALEJANDRO - University Of Barcelona
item Kuhn, David
item BARRIENTOS-PRIEGO, ALEJANDRO - University Of Chapingo
item SALOJARVI, JARKKO - Nanyang Technological University
item LIBRADO, PABLO - Natural History Museum Of Denmark
item SANKOFF, DAVID - University Of Ottawa
item HERRERA-ESTRELLA, ALFREDO - National Laboratory Of Genomics And Biodiversity
item ALBERT, VICTOR - University Of Buffalo
item HERRERA-ESTRELLA, LUIS - National Laboratory Of Genomics And Biodiversity

Submitted to: Proceedings of the National Academy of Sciences(PNAS)
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/25/2019
Publication Date: 6/2/2019
Citation: Rendon-Anaya, M., Ibarra-Laclette, E., Mendez-Bravo, A., Lan, T., Zheng, C., Carretero-Paulet, L., Perez-Torres, C., Chacon-Lopez, A., Hernandez-Guzman, G., Chang, T., Farre, K.M., Barbazuk, W., Chamala, S., Mutwil, M., Shivhare, D., Mitter, N., Hayward, A., Fletcher, S., Rozas, J., Sanchez-Gracia, A., Kuhn, D.N., Barrientos-Priego, A.F., Salojarvi, J., Librado, P., Sankoff, D., Herrera-Estrella, A., Albert, V.A., Herrera-Estrella, L. 2019. The avocado genome enlightens deep angiosperm phylogeny and reveals pathogen-influenced gene space. Proceedings of the National Academy of Sciences. https://doi.org/10.1101/654285.
DOI: https://doi.org/10.1101/654285

Interpretive Summary: The United States is the number one importer of mango, most coming from Mexico. Prior to importation, mango must be given a hot water treatment of 30 minutes in 65°C water. It is well known that this treatment has deleterious effects on mango flavor, rate of ripening and shelf life. Polygalacturonases (PG) are enzymes that break down cell walls. This is a natural part of fruit ripening. This study compares the induction and activity of PG during ripening in hot water treated and untreated mango. Only some of the PG are transcriptionally activated by hot water treatment and contribute to the faster ripening and reduced shelf life due to the treatment. Using genomics approaches, these PG have been identified. This research will be useful to commercial producers of mango, importers and mango consumers.

Technical Abstract: Mango (Mangifera indica L.) is an important commercial fruit that shows a noticeable loss of firmness during ripening. Polygalacturonase (PG, E.C. 3.2.1.15) is a crucial enzyme for cell wall loosening during fruit ripening since it solubilizes pectin; therefore, PG activity correlates with fruit softening. Mango PGs were mapped to a genome draft from previously identified PGs from mango transcriptomes. From RNA-seq we have previously reported seventeen PGs whereas from in-depth genome analysis we identified 48 bonafide PGs. Phylogenetic analysis of the 48 mango PGs suggests that they are related to Citrus sinensis, which may indicate a recent evolutive divergence and related functions with orthologs in the tree. Expression analysis and total enzymatic activity were performed for PG transcripts expressed in mango cv. Kent mesocarp during postharvest ripening and nine PGs were differentially expressed during fruit ripening. According to the expression data, MiPG21-1, MiPG14, MiPG69-1, MiPG17, MiPG49, MiPG23-3, MiPG22-7 and MiPG16 were highly up-regulated during fruit ripening. These results correlate with a drastic loss of firmness observed in mangoes at day 10 during post-harvest, between the ethylene production burst and the climacteric peak. MiPG69-1 and MiPG23-3 had very high expression levels at day 16 of post-harvest storage, and those could be related to senescence; as well as MiPG17 and MiPG49, but with lower values.