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ARS Home » Northeast Area » Boston, Massachusetts » Jean Mayer Human Nutrition Research Center On Aging » Research » Publications at this Location » Publication #361466

Research Project: Nutrients, Aging, and Musculoskeletal Function

Location: Jean Mayer Human Nutrition Research Center On Aging

Title: Fecal menaquinone profiles of overweight adults are associated with gut microbiota composition during a gut microbiota-targeted dietary intervention

Author
item Karl, J - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item Fu, Xueyan - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item Wang, Xiaoxin - Shanghai Jiaotong University
item Zhao, Yufeng - Shanghai Jiaotong University
item Shen, Jian - Shanghai Jiaotong University
item Zhang, Chenhong - Shanghai Jiaotong University
item Wolfe, Benjamin - Tufts University
item Saltzman, Edward - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item Zhao, Liping - Shanghai Jiaotong University
item Booth, Sarah - Jean Mayer Human Nutrition Research Center On Aging At Tufts University

Submitted to: American Journal of Clinical Nutrition
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/4/2015
Publication Date: 5/27/2015
Citation: Karl, J.P., Fu, X., Wang, X., Zhao, Y., Shen, J., Zhang, C., Wolfe, B.E., Saltzman, E., Zhao, L., Booth, S.L. 2015. Fecal menaquinone profiles of overweight adults are associated with gut microbiota composition during a gut microbiota-targeted dietary intervention. American Journal of Clinical Nutrition. 102:84-93. https://doi.org/10.3945/ajcn.115.109496.
DOI: https://doi.org/10.3945/ajcn.115.109496

Interpretive Summary: Emerging evidence supports novel roles for vitamin K in cardiometabolic health, some of which may be unique to the bacterially-synthesized vitamin K forms known as menaquinones. However, factors influencing menaquinone biosynthesis and associations with cardiometabolic health have not been examined. The objective of this study was to identify associations between fecal menaquinone profiles, gut microbiota composition, and biomarkers of cardiometabolic health. Menaquinone profile and gut microbiota structure were periodically measured in fecal samples collected from seventy-seven overweight Chinese adults consuming a prescribed diet previously shown to alter gut microbiota composition and improve cardiometabolic biomarkers. Distribution of menaquinones within individual fecal samples separates individuals into two distinct groups herein introduced as menaquinotypes of the human gut. Menaquinotypes were characterized by differences in menaquinone forms and by differences in the relative abundance of bacteria at the species-level. Fecal concentrations of three menaquinone forms decreased during the intervention, as did the relative abundance of certain bacteria at the species level. Neither individual menaquinones nor menaquinotypes were longitudinally associated with markers of insulin resistance, or inflammation. These findings suggest that variability in fecal menaquinones content is predominantly determined by relatively few genera within the gut microbiota. It also suggests that diet-mediated alterations in gut microbiota composition may provide a feasible approach for altering gut menaquinones content.

Technical Abstract: Background: Emerging evidence supports novel roles for vitamin K in cardiometabolic health, some of which may be unique to the bacterially synthesized vitamin K forms known as menaquinones. However, factors influencing menaquinone biosynthesis by the gut microbiota and associations with cardiometabolic health have not been examined. Objective: The objective of this study was to identify associations between fecal menaquinone profiles, gut microbiota composition, and biomarkers of cardiometabolic health. Design: The menaquinone profile and gut microbiota structure were periodically measured in fecal samples collected from 77 overweight Chinese adults who consumed a prescribed diet previously shown to alter gut microbiota composition and to improve cardiometabolic biomarkers. Results: Covariance among menaquinones within individual fecal samples partitioned individuals into 2 distinct groups, herein introduced as menaquinotypes of the human gut. Menaquinotypes were characterized by differences in menaquinone (MK) 5 and MK9-MK13 and differences in the relative abundance of several operational taxonomic units (OTUs) delineated at the species level, predominantly within the genera Prevotella spp. and Bacteroides spp. Fecal MK4, MK6, and MK8 decreased during the intervention (P<0.05); and longitudinal changes in the relative abundance of >100 OTUs were associated with altered fecal content of >/= 1 individual menaquinone. The strongest and most consistent relations were between Prevotella spp. and MK5 and MK11-MK13, between Bacteroides spp. and MK9 and MK10, and between Escherichia/Shigella spp. and MK8. Neither individual menaquinones nor menaquinotypes were longitudinally associated with markers of glycemia, insulin resistance, or inflammation. Conclusions: These findings suggest that variability in fecal menaquinone content is predominantly determined by relatively few genera within the gut microbiota and that diet-mediated alterations in gut microbiota composition may provide a feasible approach for altering gut menaquinone content. This trial was registered at the Chinese Clinical Trials Registry as ChiCTR-TRC-09000353.