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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #361388
Research Project: Molecular Identification and Characterization of Bacterial and Viral Pathogens Associated with Foods

Location: Produce Safety and Microbiology Research

Title: Top-down (and middle-down) proteomics of bacterial proteins and toxins using MALDI-TOF-TOF-MS/MS

Author
item Fagerquist, Clifton - Keith

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 3/31/2019
Publication Date: 4/2/2019
Citation: Fagerquist, C.K. 2019. Top-down (and middle-down) proteomics of bacterial proteins and toxins using MALDI-TOF-TOF-MS/MS. Meeting Proceedings. Mass Spectrometry: Application to the Clinical Lab 2019, March 31-April 4, 2019, Palm Springs, CA.

Interpretive Summary:

Technical Abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is now routinely used for taxonomic identification of bacteria at the genus and species level (and in some cases at the sub-species level). In addition, MALDI-time-of-flight-time-of-flight (MALDI-TOF-TOF) instruments can be used to identify specific protein ion peaks by tandem mass spectrometry (MS/MS). The fragment ions generated by MS/MS can be compared to a database of in silico fragment ions of bacterial protein sequences (derived from genomic sequencing) that allow identification of both the protein as well as the microorganism that generated it. This method of protein/microorganism identification is particularly useful for the rapid identification of bacterial protein toxins.