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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #360969

Research Project: Identification of Novel Management Strategies for Key Pests and Pathogens of Grapevine with Emphasis on the Xylella Fastidiosa Pathosystem

Location: Crop Diseases, Pests and Genetics Research

Title: Characterization and detection of grapevine fungal pathogens using Fatty Acid Methyl Ester analyses (FAME)

item Wallis, Christopher

Submitted to: International Workshop on Grapevine Trunk Diseases
Publication Type: Abstract Only
Publication Acceptance Date: 2/28/2019
Publication Date: 7/7/2019
Citation: Wallis, C.M. 2019. Characterization and detection of grapevine fungal pathogens using fatty acid Methyl Ester analyses (FAME). International Workshop on Grapevine Trunk Diseases. p. 43.

Interpretive Summary:

Technical Abstract: Grapevines can become infected by a variety trunk disease-causing fungi including those from genera such as Diplodia, Neofusicoccum, Lasiodiplodia, and others. These diverse pathogens can vary greatly in disease severity and symptom expression. Therefore, proper identification is necessary to determine the best management strategy. Although sequence-based genotyping can distinguish these fungal canker pathogens, there is a need for faster, cheaper ways to distinguish casual agents of trunk diseases. To address this, fingerprinting of fatty acids that comprise cell membranes of each pathogen was performed using gas chromatography on methyl esters (FAME). This was done to create profiles for over 20 different fungal species, with additional analyses that followed to verify accuracy of identification. With FAME, a total of 20 samples from culture can be analyzed in as little as two hours, and costs far lower than that of DNA-based techniques at less than 10 cents a sample. Ongoing work is examining ways to extract and analyze fungal FAMEs directly from plant tissues to avoid the need for culturing. FAME allows an alternative to DNA-based identification and could be employed in cases where nucleic acid degradation is a concern, or if an alternative methodology is required for regulatory actions.