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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #360618
Research Project: Gene Discovery and Designing Soybeans for Food, Feed, and Industrial Applications

Location: Plant Genetics Research

Title: Draft genome sequence of bradyrhizobium sp. Strain LVM 105, a nitrogen-fixing symbiont of chamaecrista fasciculata (michx) greene

Author
item Krishnan, Hari
item KIM, WON-SEOK - University Of Missouri
item GIVAN, SCOTT - University Of Missouri

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/5/2019
Publication Date: 4/4/2019
Citation: Krishnan, H.B., Kim, W., Givan, S.A. 2019. Draft genome sequence of bradyrhizobium sp. Strain LVM 105, a nitrogen-fixing symbiont of chamaecrista fasciculata (michx) greene. Microbiology Resource Announcements. 8(14):e00132-19. https://doi.org/10.1128/MRA.00132-19.
DOI: https://doi.org/10.1128/MRA.00132-19

Interpretive Summary: Soil-bacteria belonging to the genera Bradyrhizobium and Sinorhizobium can elicit nodules on the roots of soybean and other legumes. The nodules are specialized organs where atmospheric nitrogen is fixed by the bacteria. The fixed nitrogen is utilized by soybean plants for its growth and development. This process is termed biological nitrogen fixation and it enables legumes to thrive in nitrogen-poor soils. Recently, we have isolated a soil bacterium Bradyrhizobium sp. LVM 105, that forms nitrogen-fixing nodules on partridge pea. This strain also forms nitrogen-fixing nodules on some primitive soybean cultivars but not on advanced North American soybean cultivars. The reason why this bacteria does not nodulate advanced soybean cultivars remains poorly understood. We have elucidated the genome sequence of Bradyrhizobium sp. LVM 105. Such information is required for designing strategies targeted toward improving biological nitrogen fixation. Information obtained from the genome sequence of LVM 105 will help to better understand the factors that limit the formation of nitrogen-fixing nodules on North American soybean cultivars. The information obtained from this study will benefit the US soybean farmers by providing tools that will increase the soybean yields with minimal use of nitrogen fertilizers.

Technical Abstract: Partridge pea, a native North American prairie and savannah annual legume, forms nodules in association with relatively uncharacterized Bradyrhizobium species. Here, we present the draft genome sequence of Bradyrhizobium sp. LVM 105, a soil bacterium that forms nitrogen-fixing nodules on the roots of Partridge pea. The genome size was 8,386,928 bp with a GC content of 63.8%. The draft assembly contained 177 scaffolds with an N50 of 415,026 bp.