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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #360339

Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Molecular characterization and detection of two novel carlaviruses infecting cactus

Author
item Peng, Liang - Nanchang University
item Wu, Liping - Nanchang University
item Grinstead, Sam
item Kinard, Gary
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/10/2019
Publication Date: 5/10/2019
Citation: Peng, L., Wu, L., Grinstead, S.C., Kinard, G.R., Li, R. 2019. Molecular characterization and detection of two novel carlaviruses infecting cactus. Archives of Virology. https://doi.org/10.1007/s00705-019-04279-w.
DOI: https://doi.org/10.1007/s00705-019-04279-w

Interpretive Summary: The cactus family (Cactaceae) includes ornamental, food and medicinal plants. Many viruses infect cactus plants, causing various diseases. In this study, two new viruses were identified in an ornamental orchid cactus using a sensitive sequencing technology. The complete genomic sequences of these viruses were determined. Analyses of the genetic information show both are new and different viruses in the betaflexivirus group. The viruses were also detected from other cactus species. The study provides the information necessary to classify the viruses, and to help develop detection methods for them.

Technical Abstract: Two large contigs with sequence similarities to different carlaviruses were identified by high-throughput sequencing from a cactus plant. The complete genomes of the two viruses, tentatively named as cactus carlavirus 1 (CCV-1) and CCV-2, were determined to be 8,441 and 8, 396 nucleotides long, respectively, excluding the poly (A) tail. These viruses have the typical genomic organization of members of the genus Carlavirus. The sequence identities between these viruses and other carlarviruses are 48.9-60.0% at whole genome sequences. Phylogenetic analyses place the two viruses in a subgroup. The data support that both viruses are new members of the genus Carlavirus.