Location: Animal Disease ResearchTitle: Pattern of CD14, CD16, CD163 and CD172a expression on water buffalo (Bubalus bubalis) leukocytes
|ELNAGGAR, MAHMOUD - Washington State University|
|GRANDONI, FRANCESCO - Crea|
|ABDELLRAZEQ, GABER - Washington State University|
|EL-NAGGAR, KARIMA - University Of Alexandria|
|HULUBEI, VICTORY - Washington State University|
|BUTTAZZONI, LUCA - Crea|
|KHALIEL, SAMY - University Of Alexandria|
|TORKY, HELMY - University Of Alexandria|
|DAVIS, WILLIAM - Washington State University|
Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/22/2019
Publication Date: 3/25/2019
Citation: Elnaggar, M.M., Grandoni, F., Abdellrazeq, G.S., Fry, L.M., El-Naggar, K., Hulubei, V., Buttazzoni, L., Khaliel, S.A., Torky, H.A., Davis, W.C. 2019. Pattern of CD14, CD16, CD163 and CD172a expression on water buffalo (Bubalus bubalis) leukocytes. Veterinary Immunology and Immunopathology. 211:1-5. https://doi.org/10.1016/j.vetimm.2019.03.010.
Interpretive Summary: Studies on the immune response in species other than humans and mice remain constrained by gaps in the monoclonal antibody reagents available for research, especially for cattle, buffalo, sheep, goats, and swine. An objective of our ongoing research program has been to address this problem by developing monoclonal antibody where there is a critical need. In this context, we tested several monoclonal antibodies known to react with certain markers on different white blood cells from cattle, and confirmed that they cross-react with similar markers on the white blood cells of buffalo. These antibodies can now be used to aid in studying the immune response of buffalo to various important viral, bacterial, and parasitic pathogens that cause significant losses to the industry.
Technical Abstract: Previous studies on the immune system of water buffalo (Bubalus bubalis) using cross-reactive monoclonal antibodies (mAbs) revealed significant similarities and differences to the bovine immune system. Herein, we extend these studies and document the pattern of expression of CD14, CD16, CD163A and CD172a on buffalo leukocytes using a set of cross-reactive mAbs that are known to recognize conserved epitopes within orthologous molecules in cattle, sheep and goats. Buffalo leukocytes were isolated and subjected to mAb labelling for flow cytometry. Single color flow cytometry confirmed mAb recognition of buffalo orthologues of CD14, CD16, CD163A and CD172a, and revealed an expression pattern similar to that observed in other ruminants. Multicolor flow cytometry revealed that buffalo monocytes uniquely co-express CD14, CD16, CD163A and CD172a, whereas buffalo granulocytes co-express only CD16 and CD172a. This study expands mAbs available to study the innate immune response of buffalo.