|HAJERI, SUBHAS - Central California Tristeza Eradication Agency|
|LIAO, WEI - Central California Tristeza Eradication Agency|
|WESTERMAN, KAREN - Central California Tristeza Eradication Agency|
|BARNIER, JILL - Central California Tristeza Eradication Agency|
|LUO, WEIQI - North Carolina State University|
|Yokomi, Raymond - Ray|
Submitted to: International Research Conference on Huanglongbing
Publication Type: Abstract Only
Publication Acceptance Date: 2/8/2019
Publication Date: 3/10/2019
Citation: Hajeri, S., Liao, W., Westerman, K., Barnier, J., Luo, W., Gottwald, T.R., Yokomi, R.K. 2019. High-risk based field survey and high-throughput qPCR detection system for Huanglongbing-associated bacteria in commercial groves in California. International Research Conference on Huanglongbing. p. 5-61.
Technical Abstract: Citrus is a major economic crop of California with an impact of over $7 billion. In California, ‘Candidatus Liberibacter asiaticus’ (CLas) is associated with huanglongbing (HLB), transmitted by Asian citrus psyllid (ACP), and is a serious threat to the citrus industry. More than 900 CLas-positive trees in southern California have been identified and removed by California Department of Food and Agriculture (CDFA) since first detected in 2012. All finds have been in dooryard trees and none in commercial citrus production to date. Working in collaboration with ARS, the CCTEA’s Citrus Pest Detection Program (CPDP) adopted high throughput real-time PCR procedures and a high-risk based perimeter survey system to test for CLas, Citrus tristeza virus (CTV), and Spiroplasma citri. With these procedures, the CCTEA obtained a CDFA Plant Pest Permit to test non-regulatory samples for CLas and have tested more than 4,000 ACP and 30,000 plant samples for CLas in 2018. Therefore, this program can play a significant role in large-scale insect and plant sample screening for CLas, CTV and S. citri.