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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #359314

Research Project: Novel Methods for Controlling Trichothecene Contamination of Grain and Improving the Climate Resilience of Food Safety and Security Programs

Location: Mycotoxin Prevention and Applied Microbiology Research

Title: Enrichment cultures with the ability to biotransform deoxynivalenol

Author
item Shanakhat, Hina - University Of Naples
item Mccormick, Susan
item Bakker, Matthew

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/4/2018
Publication Date: 12/4/2018
Citation: Shanakhat, H., McCormick, S.P., Bakker, M.G. 2018. Enrichment cultures with the ability to biotransform deoxynivalenol [abstract].

Interpretive Summary:

Technical Abstract: The production and accumulation of trichothecene mycotoxins is responsible for much of the negative economic impact associated with Fusarium head blight. A variety of biochemical transformations to trichothecenes have been described, some of which result in a less toxic product. We expect that continued prospecting will reveal additional microbial transformations to trichothecenes, and eventually microbial enzymes having utility in plant protection or in restoring value to contaminated grain. We have developed methods for producing enrichment cultures in which complex microbial consortia (e.g., seeded from soil dilutions) are directed towards the transformation of deoxynivalenol (DON). We can now reliably produce enrichment cultures that transform DON. However, deriving from these communities a pure culture of an organism that transforms DON has remained elusive. Here we describe our procedure for producing enrichment cultures, and the microbial communities that develop in these enrichment cultures over time.