|SHANAKHAT, HINA - University Of Naples|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/4/2018
Publication Date: 12/4/2018
Citation: Shanakhat, H., McCormick, S.P., Bakker, M.G. 2018. Enrichment cultures with the ability to biotransform deoxynivalenol [abstract].
Technical Abstract: The production and accumulation of trichothecene mycotoxins is responsible for much of the negative economic impact associated with Fusarium head blight. A variety of biochemical transformations to trichothecenes have been described, some of which result in a less toxic product. We expect that continued prospecting will reveal additional microbial transformations to trichothecenes, and eventually microbial enzymes having utility in plant protection or in restoring value to contaminated grain. We have developed methods for producing enrichment cultures in which complex microbial consortia (e.g., seeded from soil dilutions) are directed towards the transformation of deoxynivalenol (DON). We can now reliably produce enrichment cultures that transform DON. However, deriving from these communities a pure culture of an organism that transforms DON has remained elusive. Here we describe our procedure for producing enrichment cultures, and the microbial communities that develop in these enrichment cultures over time.