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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Genetics, Breeding, and Animal Health Research » Research » Publications at this Location » Publication #359180

Research Project: Developing a Systems Biology Approach to Enhance Efficiency and Sustainability of Beef and Lamb Production

Location: Genetics, Breeding, and Animal Health Research

Title: Host genetics help shape the rumen microbiome in beef cattle

Author
item ABBAS, WASEEM - University Of Nebraska
item HOWARD, JEREMY - University Of Nebraska
item PAZ, HENRY - Mississippi State University
item Hales, Kristin
item Wells, James - Jim
item Kuehn, Larry
item SPANGLER, MATTHEW - University Of Nebraska
item ERICKSON, GALEN - University Of Nebraska
item FERNANDO, SAMODHA - University Of Nebraska

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 12/11/2018
Publication Date: 7/29/2019
Citation: Abbas, W., Howard, J.T., Paz, H.A., Hales, K.E., Wells, J., Kuehn, L.A., Spangler, M.L., Erickson, G.E., Fernando, S.C. 2019. Host genetics help shape the rumen microbiome in beef cattle. Journal of Animal Science. 97(Supplement 2):51. https://doi.org/10.1093/jas/skz122.093.
DOI: https://doi.org/10.1093/jas/skz122.093

Interpretive Summary:

Technical Abstract: In this study, we investigated the degree to which host genetics shape the rumen microbiome. Complex and diverse microbial communities can alter the nutrient profile available to the animal and subsequent performance. Studies of the gut microbiome have demonstrated that host genotype influences gut microbial species composition. Therefore, microbial species composition in the rumen may be a complex trait that manifests through the convergence of host-genetics and environmental factors. To test this hypothesis, we collected rumen contents and blood samples from 586 beef cattle on different diets from two locations. The rumen samples were used to sequence the V4 region of the 16S rDNA on an Illumina MiSeq platform. Animals were genotyped with various platforms and a common set of 61,974 SNP were used to conduct a genome-wide association study (GWAS) using the microbiome as a response variable. The GWAS was performed using Bayesian GBLUP fitting fixed effects of cohort (location and date), and the first 2 principle components to account for population stratification. Median posterior genomic heritability estimates were 0.110, 0.124, and 0.141 at the OTU, family and phylum taxonomic level, respectively. The top 1-Mb windows for OTUs (n=8) on 7 different chromosomes were identified. These regions affect the rumen microbiota in multiple ways; some (chromosome 19; position 3.0-4.0 Mb) are associated with closely related taxa (Prevotellaceae, Paraprevotellaceae, and RF16), some (chromosome 27; position 3.0-4.0 Mb) are associated with distantly related taxa (Prevotellaceae, Fibrobacteraceae, RF16, RFP12, S24-7, Lentisphaerae, and Tenericutes) and others (chromosome 23; position 0.0-1.0) controlling both related and unrelated taxa. Overall, the 8 regions identified control 11 different families and 6 different phyla. This study shows that host genetics can affect rumen bacterial community members and points towards the possibility that genomics can be used to manipulate the rumen microbiome.