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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Agricultural Genetic Resources Preservation Research » Research » Publications at this Location » Publication #358519

Research Project: Efficient and Effective Preservation and Management of Plant and Microbial Genetic Resource Collections

Location: Agricultural Genetic Resources Preservation Research

Title: Cryobanking, preservation of potato genetic resources. (Presentation at CSU Extension, San Luis , CO)

item Jenderek, Maria
item Hall, Bradford
item TANNER, JUSTIN - Colorado State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/7/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary: N/A

Technical Abstract: Potato genetic resources are curated at the USDA-ARS, Potato Germplasm Introduction Station at Sturgeon Bay, WI. The station holds 5,966 accessions in 92 taxa, out of which ca. 1,000 accessions are kept in vitro. The Station evaluates, maintains and distributes the germplasm to qualified customers for research and cultivar development. Mid- and long-term preservation is carried out in the USDA-ARS, National Laboratory for Genetic Resources Preservation at Fort Collins, CO. Both institutions are part of the USDA-ARS, National Plant Germplasm System (NPGS) that safeguards agricultural genetic resources for current and future generations. The Colorado genebank preserves the NPGS genetic resources and also Plant Variety Protection (PVP) voucher specimens. Mid-term storage is done by mini-tubers that usually kept in vitro for about four years (5oC, dark) without the culture transfers. This type of storage allows for a rapid culture distribution. Long-term preservation is conducted in liquid nitrogen (LN, -196oC) and requires defined processing procedures that support the material survival in the ultra-low temperature. In this kind of preservation, meristem shoot tips (0.4-1 mm) are preconditioned and exposed to a standard plant vitrification solution (PVS2) and stored into LN. Only material with >40% viability, after exposure, is stored in the LN vault. The efficacy of the processed material is ca. 98%. Currently, the NLGRP stores >430 potato accessions in LN and mid-term storage.