Submitted to: Journal of Liquid Chromatography and Related Technologies
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/31/2018
Publication Date: 1/2/2019
Citation: Appell, M., Evans, K.O., Jackson, M.A., Compton, D.L. 2019. Determination of ochratoxin A in grape juice and wine using nanosponge solid phase extraction clean-up and liquid chromatography with fluorescence detection. Journal of Liquid Chromatography and Related Technologies. 41:949-954. https://doi.org/10.1080/10826076.2018.1544148.
Interpretive Summary: Ochratoxin A is a toxic natural product that can contaminate a wide variety of foods and beverages, including fruit juices. A material to selectively isolate ochratoxin A from beverages was designed using computational chemistry techniques. The material was synthesized and then characterized by spectroscopic methods. The features of the material were observed on the nanoscale. The material removed components of samples that interfered with detection and improved detection accuracy. The results of this study will be useful to researchers looking for convenient methods to detect ochratoxin A in beverages.
Technical Abstract: Ochratoxin A is mycotoxin that can contaminate a variety of agricultural commodities, including fruit juices and wines. A detection method of ochratoxin A in grape juice and wine is reported that utilizes b-cyclodextrin, polyurethane nanosponge materials for solid phase extraction clean-up of beverage samples. Infrared analysis indicated a highly cross-linked structure that correspond with vibrational frequency analysis of PM6 semi-empirical computational models. Analysis of the material through atomic force microscopy (AFM) indicated nano-sized morphological features. A liquid chromatography method using fluorescence detection (LC-FLD) was capable of detecting ochratoxin A levels below regulatory levels in grape juice and wine. The recoveries of spiked ochratoxin A (0.5–20 ng mL_1) were between 77.0–89.4% in wine and 69.1–86.5% in grape juice.