|Appala, Keerthi - Western Kentucky University|
|Kasumba, John - Western Kentucky University|
|Conte, Eric - Western Kentucky University|
|Carlisle, Anne - Western Kentucky University|
Submitted to: Kentucky Academy of Sciences Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 10/12/2018
Publication Date: 11/3/2018
Citation: Appala, K., Kasumba, J., Conte, E., Agga, G.E., Loughrin, J.H., Carlisle, A. 2018. Quantification of tylosin antibiotics and antibiotic resistance genes in cattle waste. Kentucky Academy of Sciences Symposium. Oral Presentation.
Technical Abstract: Each year 2 million people suffer from the infections caused by bacteria, which are resistant to antibiotics, and about 23,000 people will die as a result. New drugs are coming into the market but are at the threat of developing resistance. One reason for the development of antibiotic resistance is the overuse of antibiotics in the livestock production. Tylosin is a macrolide antibiotic found naturally as a fermentation product of Streptomyces fradiae and is mainly used in promoting growth and treating infections in animals. Tylosin acts by inhibiting protein synthesis in bacteria. In cattle, tylosin is used for treating the bovine respiratory complex, foot-rot and calf diphtheria, while in swine it is used to treat swine arthritis, swine pneumonia, and swine erysipelas. The products from livestock, treated with antibiotics, such as milk, meat (Chicken, pork, cattle beef), excreta and manure pose residual antibiotics and antibiotic resistance genes (ARGs) which are consequently passed to humans. This research is focused on developing and validating a solid phase extraction (SPE) procedure and an LC-MS/MS method for quantifying tylosin in cattle waste. A sodium-EDTA buffer solution and methanol are added to the cattle waste samples. The samples are then cleaned up using Strata polymeric weak cation cartridges. Chemical analysis of the extracted tylosin is performed using a Varian 212-LC HPLC and Agilent 500 Ion Trap mass spectrometer detector. The results of this study will be presented, which include the percent recovery of tylosin in the cattle waste of tylosin treated cattle compared with a control group, which did not receive tylosin. Also, resistant genes in the cattle waste samples will be examined.