Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 9/4/2018
Publication Date: N/A
Interpretive Summary: N/A
Technical Abstract: The method of using dormant buds (DB) for germplasm cryopreservation was published a few decades ago and since then, it was used in preserving genetic resources of selected horticultural woody-plant species. The advantages of employing DB in preservation are widely known; the most relevant are no requirements of aseptic cultures, high processing throughput and involvement of a relatively low skilled technical support; but the method has also shortcomings as seasonality of processing and lack of procedural modifications that might support processing of all accessions in a collection. The U.S. NLGRP cryopreserved DB of 2,115 unique Malus accessions (in 51 species) with a >40% post cryo viability (lab standard). The method worked for all accessions in 20 species, for >90% of accessions in six species and at a various percent (0-89%) in the remaining 25 species. For species with the largest number (>88) of cryoprocessed accessions (M. domestica, M. hybr. and M. siviersii), the percent of accessions responding favorable to the DB method was high (82-97%). The results indicated variability in Malus DB response to LN exposure; similar results were recorded in the Pyrus and Ribes collections. Procedural refinements of the DB cryopreservation method might support the method applicability to a much higher number of accessions and its use in other collections of horticultural woody species. Our study on Vaccinium cryopreservation showed twig pre-harvest temperature was critical for a successful cryopreservation of DB as well as application of cryoprotectans and antioxidants in cryoprprocessing of Prunus DBs.