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Research Project: Monitoring and Molecular Characterization of Antimicrobial Resistance in Foodborne Bacteria

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Title: Draft genome sequences of two ciprofloxacin-resistant Salmonella enterica subsp. enterica serovar Kentucky ST198 isolates from retail chicken carcasses in Egypt

item RAMADAN, HAZEM - Mansoura University
item GUPTA, SUSHIM - Orise Fellow
item SHARMA, POONAM - Orise Fellow
item SALLAM, KHALID - Mansoura University
item Hiott, Lari
item ELSAYED, HAGAR - Mansoura University
item Barrett, John
item Frye, Jonathan
item Jackson, Charlene

Submitted to: Journal of Global Antimicrobial Resistance
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/24/2018
Publication Date: 6/30/2018
Citation: Ramadan, H., Gupta, S., Sharma, P., Sallam, K., Hiott, L.M., Elsayed, H., Barrett, J.B., Frye, J.G., Jackson, C.R. 2018. Draft genome sequences of two ciprofloxacin-resistant Salmonella enterica subsp. enterica serovar Kentucky ST198 isolates from retail chicken carcasses in Egypt. Journal of Global Antimicrobial Resistance. 14:101-103.

Interpretive Summary: Ciprofloxacin-resistant Salmonella serotype Kentucky was identified from human patients in Egypt at the beginning of the last decade and rapidly disseminated to other African and Middle East countries. Available information about the ciprofloxacin-resistant S. Kentucky clone from poultry and poultry products in Egypt is still limited. The overall objective of this study was to improve understanding of potential sources of human infections by ciprofloxacin-resistant S. Kentucky. Isolates displayed multidrug resistance to ciprofloxacin, ampicillin, chloramphenicol, levofloxacin, nalidixic acid, sulfisoxazole, and tetracycline; one isolate also exhibited reduced susceptibility to trimethoprim/sulfamethoxazole. Whole-genome sequence analysis identified resistance genes to those drugs. Detection of this clone on retail poultry in Egypt indicates that the clone continues to persist as a threat to public health. This information is useful for epidemiologists and regulatory agencies and warrants the implementation of national and international control strategies to limit the global spread of this strain.

Technical Abstract: Objectives: Salmonella enterica serovars, particularly drug resistant strains, pose major threats to public health worldwide. This study describes the draft genome sequences of two ciprofloxacin-resistant Salmonella enterica serovar Kentucky recovered from chicken carcass rinsates in Mansoura, Egypt. Methods: Antimicrobial resistance phenotypes were determined for the two Salmonella Kentucky by broth microdilution using the SensititreTM system. Genomic DNA from both isolates was sequenced using the Illumina MiSeq. Antimicrobial resistance genes and multilocus sequence types (MLST) were identified using ARG-ANNOT and MLST 1.8 of the Center for Genomic Epidemiology, respectively. Results: The draft genome for S. Kentucky H5 contained 4.84 Mbp in 54 contigs, and 4.94 Mbp in 64 contigs for S. Kentucky isolate H18. Sequence analysis using ARG-ANNOT identified the presence of resistance genes (blaTEM-57, aadA1, aadA2, cmlA1, sul3, and tetA) in both isolates and dfrA, sul2, floR, and aph(3’)-Ia in S. Kentucky isolate H18 only. Amino acid substitutions, Ser83Phe and Asp87Gly of gyrA, and Thr57Ser and Ser80Ile of parC, were detected in both isolates. Both isolates were belonged to sequence type 198 (ST198). Conclusion: The draft genome sequence provided identification of ciprofloxacin-resistant S. Kentucky ST198 epidemic clone with multidrug resistance in poultry products in Egypt produced for human consumption. This data indicated that poultry continues to be a reservoir for this persistent clone.