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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #356635

Research Project: Molecular and Environmental Factors Controlling Aflatoxin Reduction by Non-Toxigenic Aspergillus Strains

Location: Food and Feed Safety Research

Title: A metagenomics study of the corn kernel and silk microbiome following infection with aflatoxigenic and non-aflatoxigenic Aspergillus flavus strains

item Moore, Geromy
item CHALIVENDRA, SUBBAIAH - Louisiana State University
item Rajasekaran, Kanniah - Rajah
item Cary, Jeffrey
item Gilbert, Matthew
item Mack, Brian
item Rivers, Adam
item NGUYEN, ANTHONY - Louisiana State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/4/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: To more easily discern between the introduced A. flavus strains, K49 has been tagged with green fluorescent protein (GFP), and TOX4 with a red fluorescent protein (mCherry). Thus, we have four treatments for each corn variety as follows: Control (no spores), K49-GFP alone, TOX4-RFP alone, and mixture of K49-GFP+TOX4-RFP. Samples from corn ears will be collected throughout the growing season and prepared for metagenomics sequencing. We will have 4-5 biological replicates for three corn tissues (silk, kernel endo-biota, kernel epi-biota) that will be sampled at each of three time points.