Submitted to: Journal of Asia Pacific Entomology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/30/2018
Publication Date: 9/7/2018
Citation: Chang, C.L., Geib, S.M. 2018. Comparative proteomic profiling within each developmental stage of the solanum fruit fly, Bactrocera latifrons Hendel. Journal of Asia Pacific Entomology. https://doi.org/10.1016/j.aspen.2018.08.014.
Interpretive Summary: Although fruit fly development has been widely studied in detail, understanding of “what”, “when”, “where”, “why”, and “how” many hundred thousand proteins exist in an insect cell interact and express during development at molecular level largely remained to be clarified. We conducted proteome mapping in all developmental stage of the solanum fruit fly, Bactrocera latifrons (Hendel), by comparing all ages within a stage to their 1-d-old, using 2-D gel electrophoresis and mass spectrometry. Samples of designated ages of each stage of B.latifrons were collected, analyzed, and described. A custom peptide database, derived from a publically available de novo B.latifrons transcriptome assembly was adopted for peptide identification. Identified differentially expressed proteins (DEPs) and their putative protein functions were annotated in representative SDS gel images, charts, and tables. Based on our proteomic data, we constructed reference proteome maps which not only provide valuable information toward a comprehensive understanding of fruit fly development, but also build foundation for development of novel advanced fruit fly control techniques to support sterilization insect technique (SIT). Any epigenetic impacts due to abiotic or biotic environmental factors will be easier to be identified, manipulated, and further led to gene editing research.
Technical Abstract: Samples of 1 to 10 days old larvae, 1 to 10 days old pupae, 1 to 9 days old females and males, and 1-3 days old eggs of B.latifrons were collected and analyzed using 2-D gel electrophoresis and mass spectrometry. A custom peptide database, derived from the de novo B.latifrons whole genome assembly was adopted for peptide identification. Differentially expressed proteins were identified in each stage, using the equivalents first day of the stage as the comparison and putative protein function was annotated in representative SDS gel images, charts, and tables.