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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #356413

Research Project: Intestinal Microbial Ecology and Metagenomic Strategies to Reduce Antibiotic Resistance and Foodborne Pathogens

Location: Food Safety and Enteric Pathogens Research

Title: Complete genome sequence of Megasphaera stantonii AJH120T, isolated from a chicken cecum

item MAKI, JOEL - Orise Fellow
item Looft, Torey

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/16/2018
Publication Date: 11/8/2018
Citation: Maki, J.J., Looft, T. 2018. Complete genome sequence of Megasphaera stantonii AJH120T, isolated from a chicken cecum. Microbiology Resource Announcements. 7:e01148-18.

Interpretive Summary: Poultry production is a $40 billion a year industry in the US. Understanding how microbial communities in the poultry gut impact animal health, production, and food safety is essential to support of this industry. In this study, we describe the features of the genome of a new bacterial species (Megasphaera stantonii), which was isolated from a chicken intestinal tract. Bacteria like M. stantonii can produce chemicals beneficial to poultry, including compounds that improve intestinal health and food safety. The genomic characterization of M. stantonii provides insight into the potential functions of this organism and its relatedness to similar organisms. Studies like these improve our understanding of bacteria that are present in the chicken gut and provide valuable information that can be used to improve animal health and nutrition.

Technical Abstract: A novel bacterium, Megasphaera stantonii (strain AJH120T) was isolated from the cecum of a chicken during a screen for microorganisms using a BHI medium. PacBio and Illumina MiSeq sequencing technologies were used to produce a single, contiguous chromosome. The resulting genome sequence is 2,652,760bp long with a 52.62 percent GC content.