Integrative analysis of transcriptome and epigenome data enhances the understanding of the genetic basis underlying rumen development

Authors: LIN, SHUDAI - South China Agricultural University; FANG, LINGZHAO - University Of Maryland; Liu, Ge - George; Li, Congjun - Cj

Submitted to: International Symposium of Animal Functional Genomics
Publication Type: Abstract Only
Publication Acceptance Date: 8/20/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A well-developed, fully functional rumen ensures a diary calf to be an efficient and productive animal. A better understanding of the genetic and biological mechanism underlying the rumen development is important, yet it is poorly elucidated. In this study, A rumen primary epithelial cell culture was established. The transcriptome and four histone marks (i.e., H3K27me3, H3K27Ac, H3K4me1 and H3K9me3) were genome-wide investigated using RNA-Seq and ChIP-Seq technologies respectively. Differentially expressed genes and modified histone marks were detected under the comparison of normal cultured cells and butyrate treated cells. Integrative analysis of gene expression and histone mark modification was performed to detect genomic features that were involved in the rumen development. This study provided great insights into the genetic mechanisms underlying the rumen development and offered a general framework to integrating multi-layers data to investigate the biological and genetic basis underpinning complex traits.