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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Publications at this Location » Publication #355723
Research Project: Genomics of Livestock Pests

Location: Livestock Arthropod Pests Research

Title: Sequencing of 2 BAC clones from the Deutsch strain of cattle ticks, Rhipicephalus microplus using the Pac Bio RS II method

Author
item Bendele, Kylie
item Guerrero, Felicito

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 4/13/2018
Publication Date: 4/13/2018
Citation: Bendele, K.G., Guerrero, F. 2018. Sequencing of 2 BAC clones from the Deutsch strain of cattle ticks, Rhipicephalus microplus using the Pac Bio RS II method. . PRJNA412317, MH197480, MH197481, SAMN08863977, SRR6940837.

Interpretive Summary: Third generation DNA sequencing technology was used to sequence 2 large BAC clones containing parts of the genome of the cattle tick, Rhipicephalus microplus. The DNA was derived from 14 day old eggs from the Deutsch Texas outbreak strain reared at the USDA-ARS Cattle Fever Tick Research Laboratory, Edinburg, TX. Each corresponding dataset of assembled sequence reads has been deposited at NCBI under the BioProject accession number PRJNA412317 and the Nucleotide Database for Genomic DNA Accession Numbers MH197480 and MH197481, containing 152,987 and 130,554 nucleotides, respectively. The BioSample number for both clone sequences is SAMN08863977 and the Sequence Read Archive number is SRR6940837. USDA is an equal opportunity employer.

Technical Abstract: Pac Bio RS II DNA sequencing technology was used to sequence 2 large BAC clones (BM10J12 and BM19N14) containing parts of the genome of the cattle tick, Rhipicephalus microplus. The DNA was derived from 14 day old eggs from the Deutsch Texas outbreak strain reared at the USDA-ARS Cattle Fever Tick Research Laboratory, Edinburg, TX. Each corresponding dataset of assembled sequence reads has been deposited at NCBI under the BioProject accession number PRJNA412317 and the Nucleotide Database for Genomic DNA Accession Numbers MH197480 and MH197481, containing 152,987 and 130,554 nucleotides, respectively. Both assembled BAC sequences contain 2 unordered pieces each. The BioSample number for both clone sequences is SAMN08863977 and the Sequence Read Archive number is SRR6940837. USDA is an equal opportunity employer.