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ARS Home » Pacific West Area » Pullman, Washington » Grain Legume Genetics Physiology Research » Research » Publications at this Location » Publication #355370

Research Project: Improving Genetic Resources and Disease Management for Cool Season Food Legumes

Location: Grain Legume Genetics Physiology Research

Title: Saturation of genomic region implicated in resistance to Fusarium race 5 in chickpea

item CABALLO, C - Ifapa Centro Alameda Del Obispo
item MADRID, E - Max Planck Society
item GIL, J - Universidad De Cordoba
item Chen, Weidong
item RUBIO, J - Ifapa Centro Alameda Del Obispo
item MILAN, TERESA - Universidad De Cordoba

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/21/2019
Publication Date: 2/7/2019
Citation: Caballo, C., Madrid, E., Gil, J., Chen, W., Rubio, J., Milan, T. 2019. Saturation of genomic region implicated in resistance to Fusarium race 5 in chickpea. Molecular Breeding. 39:16.

Interpretive Summary: Fusarium wilt of chickpea is a serious biological constraint of chickpea production worldwide. The causal agent Fusarium oxysporum f. sp. is divided into eight races. Chickpea resistance to this pathogen is race-specific. Researchers have developed numerous molecular markers for the races of this pathogen. However, there has been only one marker available for resistance to race 5, and this marker has limited usefulness. This research focused on clearly defining genes for resistance to race 5. We identified six markers linked to resistance to race 5. These markers will accelerate breeding efforts to make more disease resistant chickpeas.

Technical Abstract: Fusarium oxysporum f. sp. ciceris (Foc) is the major soil-borne fungus affecting chickpea and race 5 (Foc5) is the most important in the Mediterranean basin. A gene controlling resistant reaction to Foc5 has been located on LG2 of the chickpea genetic map forming a cluster with resistance genes to other Foc races. The sequence-tagged microsatellite site (STMS) marker TA59 is tightly linked to this genomic region. In the current study our objective was to look for candidate genes related to resistance to Foc5 starting from the physical position of TA59 and taking advantage of the whole chickpea genome sequence information. We selected a set of markers, covering a region of around 25 Mbp that were genotyped in Near Isogenic Lines and Recombinant Inbred Lines. Joining data of different plant materials, it was possible to define an area of approximately 820 Kbp. One of the 26 genes annotated in the selected region (LOC101511605) could be considered a candidate gene for its possible implication in resistance reactions. Single-nucleotide polymorphism (SNP) markers selected in the target region were used to screen a collection of 32 genotypes differing in their reactions to Foc5. A cluster analysis showed four main groups, three of them grouping the resistant genotypes and the fourth the susceptible lines. Comparison between the haplotypes of representative accessions for each cluster allowed the identification of six SNPs coincident among resistant lines and different from the susceptible lines. These SNPs could be used in Markers Assisted Selection.