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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #355081

Research Project: Management of Temperate-Adapted Fruit, Nut, and Specialty Crop Genetic Resources and Associated Information

Location: National Clonal Germplasm Repository

Title: Molecular characterization of divergent Closterovirus isolates infecting Ribes species

Author
item Koloniuk, Igor - Academy Of Sciences Of The Czech Republic (ASCR)
item Thekke-veetil, Thanuja - University Of Arkansas
item Reynard, Jean-sebastien - Agroscope
item Plesko, Irena Mavric - Agricultural Institute Of Slovenia
item Pribylova, Jaroslava - Academy Of Sciences Of The Czech Republic (ASCR)
item Brodard, Justine - Agroscope
item Kellenberger, Isabelle - Agroscope
item Sarkisova, Tatiana - Academy Of Sciences Of The Czech Republic (ASCR)
item Spak, Josef - Academy Of Sciences Of The Czech Republic (ASCR)
item Lamovsek, Janja - Agricultural Institute Of Slovenia
item Massart, Sebastien - University Of Liege
item Ho, Thien - University Of Arkansas
item Postman, Joseph
item Tzanetakis, Ioannis - University Of Arkansas

Submitted to: Viruses
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/10/2018
Publication Date: 7/12/2018
Citation: Koloniuk, I., Thekke-Veetil, T., Reynard, J., Plesko, I., Pribylova, J., Brodard, J., Kellenberger, I., Sarkisova, T., Spak, J., Lamovsek, J., Massart, S., Ho, T., Postman, J.D., Tzanetakis, I. 2018. Molecular characterization of divergent Closterovirus isolates infecting Ribes species. Viruses. 10(7):369. https://doi.org/10.3390/v10070369.
DOI: https://doi.org/10.3390/v10070369

Interpretive Summary: Symptoms observed in black currants and red currants (Ribes species) let to the discovery of several new viruses that belong to the family Closteroviridae. Laboratory results determined that the same viruses were present in Ribes plants at several different locations in North America and Europe. Long-distance movement of infected plant material was the likely responsible for the wide geographic distribution of these newly described plant viruses.

Technical Abstract: Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.