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ARS Home » Midwest Area » East Lansing, Michigan » Sugarbeet and Bean Research » Research » Publications at this Location » Publication #354431
Research Project: Utilizing Genetic Diversity within Phaseolus vulgaris to Develop Dry Beans with Enhanced Functional Properties

Location: Sugarbeet and Bean Research

Title: QTL mapping of resistance to bean weevil in common bean

Author
item KAMFWA, KELVIN - University Of Zambia
item BEAVER, JAMES - University Of Puerto Rico
item Cichy, Karen
item KELLY, JAMES - Michigan State University

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/9/2018
Publication Date: 9/6/2018
Citation: Kamfwa, K., Beaver, J., Cichy, K.A., Kelly, J.D. 2018. QTL mapping of resistance to bean weevil in common bean. Crop Science. 58(6):2370-2378.
DOI: https://doi.org/10.2135/cropsci2018.02.0106

Interpretive Summary: The common bean weevil (Acanthoscelides obtectus [Say]) is a major storage pest of dry beans in tropical environments. Breeding dry bean cultivars with resistance to weevils would help to prolong the shelf-life of beans without the use of pesticides. To expand the current knowledge of genetic resistance to the common bean weevil, a genetic analysis was conducted with a common bean population of 210 lines derived from a cross of Solwezi, a susceptible bean landrace from Zambia, with breeding line AO-1012-29-3-3A that possesses resistance to weevil damage. This population was genotyped with DNA markers, and evaluated for resistance to weevil feeding and seed weight. Three genomic regions were found to be associated with resistance, one of these on Chromosome 4 was previously reported as the major source of resistance to weevil feeding. The other two are new. In addition, four genomic regions related to seed weight were identified. These findings can be used in marker-assisted breeding to accelerate development of common bean cultivars with resistance to common bean weevil.

Technical Abstract: The common bean weevil (Acanthoscelides obtectus [Say]) is an important post-harvest pest of common bean (Phaseolus vulgaris L.) seed. To expand the current knowledge of resistance to A. obtectus, a quantitative trait loci (QTL) analysis was conducted using a common bean population of 210 F4:5 recombinant inbred lines derived from a cross of Solwezi, a susceptible bean landrace from Zambia, with breeding line AO-1012-29-3-3A that possesses resistance to A. obtectus. This population was genotyped with 5398 SNP markers, and evaluated for resistance to A. obtectus and seed weight. Three QTL for resistance to A. obtectus were identified on chromosomes Pv04 (AO4.1SA and AO4.2SA) and on Pv06 (A06.1SA). The AO4.1SA QTL was previously reported as the Arcelin, Phytohemagglutinin and Alpha-amylase, (APA) resistance locus on Pv04. The other two QTL for resistance to A. obtectus, AO4.2SA and AO6.1SA are new. In addition, four QTL for seed weight were identified on Pv04 (SW4.1SA), Pv07 (SW7.1SA), Pv08 (SW8.4SA), and Pv09 (SW9.4SA). Interestingly, the AO4.1SA and SW4.1SA QTL for A. obtectus resistance and seed weight, respectively, co-localized on Pv04. Once markers linked to the identified QTL for resistance are validated, they could potentially be used in marker-assisted breeding to accelerate development of common bean cultivars with resistance to A. obtectus.