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ARS Home » Midwest Area » Bowling Green, Kentucky » Food Animal Environmental Systems Research » Research » Publications at this Location » Publication #354235

Research Project: Developing Safe, Efficient and Environmentally Sound Management Practices for the Use of Animal Manure

Location: Food Animal Environmental Systems Research

Title: Quantification of tylosin and tylosin antibiotic resistance genes in cattle waste

item APPALA, KEERTHI - Western Kentucky University
item CONTE, ERIC - Western Kentucky University
item KASUMBA, JOHN - Western Kentucky University
item Agga, Getahun
item Loughrin, John

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/2/2018
Publication Date: 3/24/2018
Citation: Appala, K., Conte, E., Kasumba, J., Agga, G.E., Loughrin, J.H. 2018. Quantification of tylosin and tylosin antibiotic resistance genes in cattle waste. Meeting Abstract. Paper No. 17, p. 7.

Interpretive Summary:

Technical Abstract: Presented is the development of a solid phase extraction (SPE) procedure and a liquid chromatography-mass spectrometry (LC-MS/MS) method for quantifying tylosin in cattle waste samples. Tylosin is a macrolide antibiotic found naturally as a fermentation product of Streptomyces fradiae and is mainly used in promoting growth and treating infections in animals. Tylosin acts by inhibiting protein synthesis in bacteria. In cattle, tylosin is used for treating bovine respiratory complex, foot-rot and calf diphtheria, while in swine it is used to treat swine arthritis, swine pneumonia and swine erysipelas. The products from antibiotic treated livestock, such as milk, meat (chicken, pork, cattle beef), excreta and manure possess residual antibiotics and resistance genes (ARG’s) which are consequently passed to humans. Each year 2 million people suffer and about 23,000 die from infections caused by bacteria that are resistant to antibiotics. New drugs are coming into the market almost daily, but developing renewed resistance is a real problem. One of the reasons for the development of antibiotic resistance is the overuse of antibiotics such as tylosin in animal feed. Furthermore, tylosin resistant genes are also being measured in these waste samples by PCR (Polymerase Chain Reaction).