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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety & Processing Research » Research » Publications at this Location » Publication #354129

Research Project: Novel Pre-harvest Interventions and Alternatives to Antibiotics to Reduce Foodborne Pathogens

Location: Poultry Microbiological Safety & Processing Research

Title: Development of a loop-mediated isothermal amplification method for rapid campylobacter jejuni detection

Author
item Yeh, Hung-yueh
item Telli, Arife - Selcuk University

Submitted to: International Association for Food Protection
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary: none

Technical Abstract: Introduction: Campylobacter jejuni is the leading foodborne pathogen that causes human bacterial gastroenteritis worldwide. Poultry products are regarded as a major source for human infection. Early, rapid detection of this microorganism in poultry products is necessary for contamination control and reduction of economic losses. The loop-mediated isothermal amplification assay (LAMP) that amplifies DNA at an isothermal temperature with specificity and rapidity may be an alternative for C. jejuni detection in poultry products. Purpose: The purpose of this communication was to evaluate LAMP for rapid detection of C. jejuni. Methods: A set of four primers were designed specifically to recognize the C. jejuni potassium transport ATPaseA (kta) gene. Genomic DNA was prepared by a genomic DNA isolation kit. Amplification was performed at 65 oC for an hour. The amplicons were analyzed by agarose gel electrophoresis, and the gels were stained with ethidium bromide. Results: The results show the ladder-like patterns of the amplicons specific to the kta gene. The LAMP reaction conditions were optimized. The detection limit of this assay was about 1 pg/reaction. Significance: Based on our preliminary results, we concluded that this assay is rapid, sensitive and specific for identification of C. jejuni, and does not require sophisticated equipment. Further evaluation of LAMP on C. jejuni detection in complex food matrix is needed.