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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #353961

Research Project: Umbrella Project for Food Safety

Location: Warmwater Aquaculture Research Unit

Title: Complete genome sequence of multidrug-resistant Edwardsiella ictaluri strain MS-17-156

item ABDELHAMED, HOSSAM - Mississippi State University
item TEKEDAR, HASAN - Mississippi State University
item OZDEMIR, OZAN - Mississippi State University
item HSU, CHUAN-YU - Mississippi State University
item ARICK, MARK II - Mississippi State University
item KARSI, ATTILA - Mississippi State University
item LAWRENCE, MARK - Mississippi State University

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/29/2018
Publication Date: 5/31/2018
Citation: Abdelhamed, H., Tekedar, H., Ozdemir, O., Hsu, C., Arick, M., Karsi, A., Lawrence, M. 2018. Complete genome sequence of multidrug-resistant Edwardsiella ictaluri strain MS-17-156. Genome Announcements.

Interpretive Summary: Edwardsiella ictaluri is a significant pathogen of cultured fish, particularly channel catfish. Here, we present the complete and annotated genome of a multidrug resistant E. ictaluri strain MS-17-156 recovered from catfish. The genome sequence of this strain is expected to help us understand the molecular mechanism of antibiotic resistance, which would facilitate combating antibiotic resistance and promote antibiotic stewardship programs.

Technical Abstract: Edwardsiella ictaluri is a Gram-negative bacillus in the order Enterobacteriales. It is the etiologic agent of enteric septicemia of catfish (ESC), one of the most significant diseases of the commercial catfish industry in the United States. E. ictaluri strain MS-17-156 was isolated from a diseased channel catfish in 2017 from the Aquatic Diagnostic Laboratory at the College of Veterinary Medicine, Mississippi State University. E. ictaluri strain MS-17-156 was found to be resistant to tetracycline, oxytetracycline, doxycycline, florfenicol, erythromycin, chloramphenicol, streptomycin, penicillin, novobiocin, azithromycin, and spectinomycin. In this study, we reported the complete and annotated genome of E. ictaluri strain MS-17-156 using Nanopore and Illumina. The complete genome sequence of E. ictaluri strain MS-17-156 consists of one circular chromosome and two circular plasmids. pEI MS-17-156-1 plasmid contains resistance genes, floR and tetD, and this plasmid is nearly identical to previously sequenced plasmids isolated from a wide variety of Gram-negative bacteria. In-depth analysis of this genome is expected to increase understanding of the mechanisms of antibiotic resistance in E. ictaluri.