|Hoang, Nguyen - University Of California|
|Rwahnih, Maher Al - University Of California|
|Hsu, Erin - University Of California|
|Sim, Susan - University Of California|
|Golino, Deborah - University Of California|
Submitted to: In Vitro Cellular and Developmental Biology - Animals
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/2018
Publication Date: 4/30/2018
Citation: Hoang, N.H., Rwahnih, M., Preece, J.E., Hsu, E., Sim, S., Golino, D. 2018. Development of a meristem-tip culture procedure for eradication of cherry virus-a in selected cultivars of cherr. In Vitro Cellular and Developmental Biology - Animals. Vol.54, S30-S31.
Technical Abstract: Cherry virus A (CVA), a recently-discovered asymptomptic virus that belongs to the genus Capillovirus, appears to be wide spread in many commercial cherry cultivars. Although the effects of this virus are not yet established, the movement of cherry germplasm, even from elite collections, between States can be restricted if the virus is known to infect the material. Movement of cherry stocks between California, Oregon, and Washington has been impeded due to regulatory exclusion of this wide-spread virus. Work was conducted to optimize a meristem-tip culture procedure for eradication of CVA in selected Prunus cultivars. Infection status of four non-symptomatic cherry cultivars (Prunus spp.) was confirmed by RT-qPCR. CVA was detected in three out of four tested cultivars. Lateral shoots from the CVA-negative cherry trees (P. lannesiana cv. ‘Krymsk ®7, or P2G9) were used for meristem establishment media comparison, while three CVA infected cherry cultivars (P. avium x P. tomentosa cv. Montmorency, P. cerasus cv. English Morello, P. serrulata cv. Pink Cloud) were used as material for CVA eradication. Meristem-tips of P2G9 (0.5-0.7 mm), from in vitro plants and field-grown trees, were established with highest succesful rates on woody plant media (Lloyd and McCown, 1981) supplemented with 0.5mg/L meta-topolin (95% success from in vitro plants and 17.5% for field-grown plants). Meristems of CVA-infected cultivars’ survival rates ranged from 45-64%. In vitro shoots of 4 cultivars were rooted in vitro using IBA-pulse (10 mg/L) in combination with dark treatment. Rooted plant were successfully transferred to soil. Although further testing are required, our preliminary testing, conducted on in vitro plants, showed 91% negative for the virus (all P. cv. Montmorency).