Location: Horticultural Crops ResearchTitle: A new, prevalent virus infects blueberry Author
|Villamor, D - University Of Arkansas|
|Martin, Robert - Bob|
|Tzanetakis, I - University Of Arkansas|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/20/2018
Publication Date: 8/13/2018
Citation: Villamor, D.E., Keller, K.E., Martin, R.R., Tzanetakis, I.E. 2018. A new, prevalent virus infects blueberry. Abstract for the North American Blueberry Research and Extension Workshop; 2018 Aug 12-15; Orono, MN.
Technical Abstract: The use of clean planting material is the most important component in virus disease management. The production and release of such material is ensured through a battery of tests including biological indexing and a panel laboratory tests. Modern technologies known as high throughput sequencing (HTS), has been shown to be superior to the current standard testing protocols in being able to detect all virus variants as well novel viruses for which no laboratory tests is available. HTS aided in the discovery of a novel luteovirus that was present in virus-tested plants. The presence of the virus was confirmed by RT-PCR using primers designed from HTS-derived sequences. The virus most closely resemble nectarine stem pitting-associated virus (NSPaV). Further analysis revealed presence of this same luteovirus in six additional blueberry selections; four of which were also classified as virus tested selections. Surveys are underway to assess the distribution of this virus across the country; preliminary results revealed the presence of the virus in fields from Washington, Oregon and Michigan. It has also been detected in several northern and southern highbush blueberry cultivars held at the USDA-ARS National Clonal Germplasm repository in Corvallis, Oregon.