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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #353426

Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Molecular characterization of a novel closterovirus identified from blackcurrant by high-throughput sequencing

Author
item Zheng, Luping - Fujian Agricultural & Forestry University
item Wu, Liping - Nanchang University
item Postman, Joseph
item Liu, Huawei - China Agricultural University
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/2/2018
Publication Date: 9/11/2018
Citation: Zheng, L., Wu, L., Postman, J.D., Liu, H., Li, R. 2018. Molecular characterization of a novel closterovirus identified from blackcurrant by high-throughput sequencing. Archives of Virology. https://doi.org/10.1007/s11262-018-1598-4.
DOI: https://doi.org/10.1007/s11262-018-1598-4

Interpretive Summary: Blackcurrant (Ribes nigrum) is a woody shrub that is widely cultivated for its nutrition-rich fruits. Several viruses and virus-like pathogens infect blackcurrant, causing various diseases that reduce yields and market value of the fruit. In this study, a new virus was identified from a blackcurrant plant using a sensitive sequencing technology. The complete genomic sequence of the virus was determined. Analyses of the genetic information showed it is a new virus in the closterovirus group, and we’ve proposed naming it blackcurrant closterovirus 1 (BCCV-1) The virus was also detected in ten other currant or gooseberry accessions from Ribes germplasm collections. This study provides the information necessary to classify the virus and to help develop detection methods for quarantine and certification programs.

Technical Abstract: Two large contigs with high sequence similarities to several closteroviruses were identified by high-throughput sequencing from a blackcurrant plant. The complete genome of this new virus was determined to be 17,320 nucleotides, which contains ten open reading frames in a similar arrangement typical of closteroviruses. Phylogenetic analysis of heat shock protein 70-like protein sequences placed the new virus with members of genus Closterovirus in the same group. The virus, which is provisionally named as Blackcurrant closterovirus 1, shares 43-45% nucleotide sequence identities with other closteroviruses and should represent a new species of the genus Closterovirus.