Skip to main content
ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #353258

Research Project: Biology, Ecology, and Genomics of Pathogenic and Beneficial Microorganisms of Wheat, Barley, and Biofuel Brassicas

Location: Wheat Health, Genetics, and Quality Research

Title: Construction of a proteome reference map and response of the wheat take-all pathogen Gaeumannomyces graminis var. tritici to 2,4-diacetylphloroglucinol

Author
item Kwon, Young Sang - Korea Institute Of Industrial Technology (KITECH)
item Jeon, Chang-wook - Kyungpook National University
item Bae, Dong-won - Gyeongsang National University
item Thomashow, Linda
item Weller, David
item Kwak, Youn-sig - Gyeongsang National University

Submitted to: Fungal Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/7/2018
Publication Date: 9/18/2018
Citation: Kwon, Y., Jeon, C., Bae, D., Thomashow, L.S., Weller, D.M., Kwak, Y. 2018. Construction of a proteome reference map and response of the wheat take-all pathogen Gaeumannomyces graminis var. tritici to 2,4-diacetylphloroglucinol. Fungal Biology. https://doi.org/10.1016/jfunbio.2018.09.001.
DOI: https://doi.org/10.1016/jfunbio.2018.09.001

Interpretive Summary: Take-all disease, caused by the fungal root pathogen Gaeumannomyces graminis var. tritici, is one of the most serious root diseases of wheat worldwide. The pathogen can survive in dead roots and the basal parts of wheat plants, and then emerge to infect the new wheat crop in the following season. In this study, a database and reference map of the proteins produced by the pathogen was constructed before and after it was exposed to 2,4-diacetylphloroglucinol (DAPG), a natural antibiotic produced by beneficial bacteria that can control the fungus in certain soils where the disease is suppressed. For mapping, a total of 240 spots was identified that represented 209 different proteins. The most abundant biological function categories among these proteins were related to carbohydrate metabolism (21%), amino acid metabolism (15%), protein folding and degradation (12%), protein translation (11%), and stress response (10%). In total, 51 proteins were regulated by treatment with the natural antibiotic DAPG. Carbohydrate metabolism, amino acid metabolism, stress response, and protein folding and degradation functions were most affected. This study provided the first extensive proteomic reference map for this important plant pathogenic fungus and represents the first time that the response of the fungus to DAPG has been characterized at the protein level.

Technical Abstract: Take-all disease, caused by Gaeumannomyces graminis var. tritici, is one of the most serious root diseases in wheat production worldwide. The pathogen can survive saprophytically as hyphae in dead roots, crowns, and tiller bases, which serve as primary inoculum sources to infect the new crop the following season. In this study, a proteomic platform based on 2-DE and MALDI-TOF/TOF MS was used to construct the first proteome database reference map of G. graminis var. tritici (Ggt) and to identify the response of the pathogen to 2,4-diacetylphloroglucinol (DAPG), which is a natural antibiotic in take-all suppressive soils produced by antagonistic Pseudomonas spp. For mapping, a total of 240 spots were identified that represented 209 different proteins. The most abundant biological function categories in the Ggt proteome were related to carbohydrate metabolism (21%), amino acid metabolism (15%), protein folding and degradation (12%), translation (11%), and stress response (10%). In total, 51 Ggt proteins were regulated by DAPG treatment. Based on gene ontology, carbohydrate metabolism, amino acid metabolism, stress response, and protein folding and degradation proteins were the ones most modulated by DAPG treatment. This study provided the first extensive proteomic reference map constructed for Ggt and was the first time that the response of Ggt to DAPG was characterized at the proteomic level.