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ARS Home » Southeast Area » Canal Point, Florida » Sugarcane Field Station » Research » Publications at this Location » Publication #353188

Research Project: Identification of Resistant Germplasm and Markers Associated with Resistance to Major Diseases of Sugarcane

Location: Sugarcane Field Station

Title: Molecular characterization of genetic basis of Sugarcane Yellow Leaf Virus (SCYLV) resistance in Saccharum spp. hybrid

Author
item Islam, Md
item Yang, Xiping - University Of Florida
item Sood, Sushma
item Comstock, Jack
item Wang, Jianping - University Of Florida

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/28/2018
Publication Date: 6/28/2018
Citation: Islam, M.S., Yang, X., Sood, S.G., Comstock, J.C., Wang, J. 2018. Molecular characterization of genetic basis of Sugarcane Yellow Leaf Virus (SCYLV) resistance in Saccharum spp. hybrid. Plant Breeding. 137(4):598-604. doi:org/10.1111/pbr.12614.
DOI: https://doi.org/10.1111/pbr.12614

Interpretive Summary: Sugarcane Yellow Leaf Virus (SCYLV) causes one of the widely spread major disease in sugarcane. Host plant resistance is the most effective, economic and environmental friendly option to control the disease. Two quantitative trait loci (QTL) were detected for SCYLV resistance accounting for 28% of the phenotypic variation using an F1 segregating population derived from cross CP95-1039 x CP88-1762 in Canal Point, Florida. The major QTL qSCYLR79 located on linkage group C79 linked with marker 3tag3804 and appears to be unique for SCYLV resistance in Saccharum Spp. hybrid. Progenies having combination of two major allele had 31% less SCYLV incidence than progenies consisting combination of major and minor allele on the genomic location of qSCYLR79 linked marker. Thus, the selection against the minor allele might potentially increase the breeding value for SCYLV resistance in sugarcane hybrid.

Technical Abstract: Sugarcane (Saccharum Spp.) produces 80% of the world's sugar along with other byproducts. The production of sugarcane is vulnerable due to infestation of sugarcane yellow leaf virus (SCYLV) worldwide. A study was conducted using an F1 segregating population derived from CP95-1039 x CP88-1762 to identify the genetic factors underlying with SCYLV resistance. The disease infection data were measured using tissue blot immunoassay after six years of exposure to the virus under natural field conditions. Genetic maps were created using genotyping by sequencing based markers for each parent separately following a pseudo test-cross approach. Two quantitative trait loci (QTL) were detected for SCYLV resistance accounting for 28% of the phenotypic variation. The major QTL qSCYLR79 located on linkage group 79 and linked with marker 3PAV3154 appears to be unique for SCYLV resistance in sugarcane. Progenies having a combination of two major alles had 31% less SCYLV incidence than progenies with a combination of major and minor alleles in the genomic region of qSCYLR79. Thus, selection against the minor allele may decrease the SCYLV incidence in sugarcane.